Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Analysis of flavonoids in the mature fruit of Vaccinium uliginosum L. of China

  • Hua, Lian Li (Graduate School of Science and Technology, Kumamoto University) ;
  • Yoshitama, Kunijiro (Graduate School of Science and Technology, Kumamoto University)
  • Published : 2006.03.30
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Abstract

In this study, a new extraction method was developed and two kinds of flavonoids were extracted from the mature fruit of Vaccinium uliginosum L. of China. These two kinds of flavonoids were analyzed by spectral and identified by high-performance liquid chromatography (HPLC) and UV/Vis. The extract of the fruit was total acid hydrolyzed. TLC chromatography was subsequently employed to identify the hydrolysate. Two kinds of aglycone flavonoids, quercetin and myricetin, were identified. At the same time PC chromatography was used to identify the monomer sugar in the flavonoids and it was verified as glucose. HPLC, UV/Vis, and Mass spectrum analyses revealed that the flavonoids were quercetin 3-monoglucosides and myricetin 3-monoglucosides.

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References

  1. Abdelhak Mansouri, Guendez Embarek, Eugene Kokkalou, Panagiotis Kefalas. (2005) Phenolic profile and antioxidant activity pedate palm fruit (Phoenix dactylifera) Food Chem. 89, 411-420. https://doi.org/10.1016/j.foodchem.2004.02.051
  2. Andrade P, Ferreres F, Amaral MT. (1997) Analysis of honey phenolic acids by HPLC, its application to honey botanical characterization. J. Liq. Chromatogr. R. T. 20, 2281-2288. https://doi.org/10.1080/10826079708006563
  3. Ferreres F, Giner JM, Toma’Ss-Barbera’n FA. (1994) A comparative study of hesperetin and methyl anthranilate as markers of the floral origin of citrus honey. J. Sci. Food Agr. 65, 371-372. https://doi.org/10.1002/jsfa.2740650316
  4. Hariri EB, Salle G, Andary C. (1991) Involvement offlavonoids in the resistance of two polarcultivars to mistletoe (Viscum album L.). Protoplasma 162, 20-26. https://doi.org/10.1007/BF01403897
  5. Kaneko T, Baba N. (1999) Protective effect of flavonoids on endothelial cells against linoleic acid hydroperoxide-induced toxocity. Biosci. Biotech. Bioch. 63, 323-328. https://doi.org/10.1271/bbb.63.323
  6. Kater F, Rovel B, Girardin M, Metche M. (1996) Fractionatin and identification of the phenolic compounds of Highbush blueberries (Vaccinium corymbosum, L.). Food Chem. 55, 35-40. https://doi.org/10.1016/0308-8146(95)00068-2
  7. Senorans FJ, Ibanez E, Cavero S, Tabera J, Reglero G. (2000) Liquid chromatographic-mass spectrometric analysis of supercritical-fluid extracts of rosemary plants. J. Chromatogr. A 870, 491-499. https://doi.org/10.1016/S0021-9673(99)00941-3
  8. Ichiyanagi T, Kashiwada Y, Ikeshiro Y, Hatano Y, Shida Y, Horie M, Matsugo S, Konishi T. (2004) Complete Assignment of Bilberry (Vaccinium myrtillus L.) Anthocyanins Separated by Capillary Zone Electrophoresis. Chem. Pharm. Bull. 52, 226-229. https://doi.org/10.1248/cpb.52.226