Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Induction of Nitric Oxide Production by Bafilomycin A1 in Mouse Leukemic Monocyte Cell Line

  • Hong, Jang-Ja (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University) ;
  • Nakano, Yasuhiro (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University) ;
  • Ohuchi, Kazuo (Laboratory of Biochemistry, Faculty of Pharmacy, Yasuda Women's University) ;
  • Kang, Young-Sook (Laboratory of Pathophysiology, College of Pharmacy, Sookmyung Women's University)
  • Published : 2006.09.25
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Abstract

In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type $(H^+)$-ATPase (V-ATPase) inhibitor bafilomycin $A_1$ at 10 and 100 nM decreased cell growth and survival as determined by 3-(4,5-dimethyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in a concentration-dependent manner. At such concentrations, bafilomycin $A_1$ induced nitric oxide (NO) production through the expression of inducible nitric oxide synthase (iNOS). The bafilomycin $A_1$-induced NO production was inhibited by the NOS inhibitor $N^G$-monomethyl-L-arginine acetate (L-NMMA). Our findings suggest that the V-ATPase inhibitor bafilomycin $A_1$ induces NO production through the expression of iNOS protein.

Keywords

References

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