Changes in Total Polyphenol, Total Flavonoid Contents and Antioxidant Activities of Hibiscus cannabin us L.

  • Ryu, Seung-Won (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Jin, Cheng-Wu (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Lee, Han-Shin (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Lee, Ji-Yeon (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Sapkota, Kumar (Department of Biotechnology, Chosun Univ.) ;
  • Lee, Beom-Goo (College of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Yu, Chang-Yeon (College of Agriculture & Life Sciences, Kangwon Natl. Univ.) ;
  • Lee, Myoung-Ku (College of Forestry Sciences, Kangwon Natl. Univ.) ;
  • Kim, Myong-Jo (College of Agriculture & Life Sciences, Kangwon Natl. Univ.) ;
  • Cho, Dong-Ha (College of Bioscience & Biotechnology, Kangwon Natl. Univ.)
  • Published : 2006.10.01

Abstract

This study purposed to look for its uses by analyzing useful substances in kenaf (Hibiscus cannabin us L.) leaves based on the total polyphenol and flavonoid contents in the leaves, DPPH radical scavenging activity of leaf extract, SOD activity, etc. by different growth stage and variety. According to the results of experiment, the total polyphenol content was highest in Everglade-41 (27.9 mg/g dw.), the total flavonoid content was highest in Tainung-2 (42.3 mg/g dw.), and SOD activity was highest in Dowling (96.1 %). However, DPPH radical scavenging activity was similar among the three varieties.

Keywords

References

  1. Agbor AG, Oben JE, Ngogang JY (2001) Effect of water extract of Hibiscus cannabinus leaves on Phenylhydrazine Induced Haemolytic anaemia. Paper presented at the 8th symposium of the Cameroon Society for Biochemistry and Molecular Biology, University of Dschang, 25-26
  2. Agbor AG, Oben JE, Ngogang JY (2002) Hepatoprotective activity of Hibiscus cannabinus leaves extract. International Symposium on Medicinal Plants, Health and Environment Book of abstracts, 24
  3. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I (2001) Antioxidant principles from Bauhinia terapotensis. Journal of Natural Prod. 64:892-895 https://doi.org/10.1021/np0100845
  4. Bradford MM (1976) A rapid and sensitive method for the quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  5. Foyer CH, Lopez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione associated mechanism of acclimatory stress tolerance and signalling. Physiol. Plant 100:241-254 https://doi.org/10.1111/j.1399-3054.1997.tb04780.x
  6. Hertog MGL, Hollman PCH (1996) Potential health effects of dietary flavonol quercetin. Eur. J. Clin. Nutr. 50:63-71
  7. Hagerman A, Harvey-Mueller I, Makkar HPS (2000) Quantification of tannins in tree folige a laboratory manual. FAO/IAEA. Vienna
  8. Hwang EJ, Lee SY, Kwon SJ, Park MH, Boo HO (2006) Antioxidative, Antimicrobial and Cytotoxic Activities of Fagopyrum esculentum Mench Extract in Germinated Seed. Korean J. Medicinal Crop Sci. 14(1):1-7
  9. Lam LKT, Zhang J, Hasegawa S (1994) Citrus Iimonoid reduction of chemically induced tumorigenesis. Food Technol. 48:104-109
  10. Lee ST, Ryu JS, Kim MB, Kim DK, Lee HJ, Heo JS (1999) Crude saponin contents of Platycodon grandiflorum (Jacg.) A. DC. Kor. J. Medicinal Crop Sci. 7:172-176
  11. Lee SY, Hwang EJ, Kim GH, Choi YB, Lim CY, Kim SM (2005) Antifungal and Antioxidant Activities of Extracts from Leaves and Flowers of Camellia japonica L. Korean J. Medicinal Crop Sci. 13(3):93-100
  12. Miller EG, Gonzales-Sanders AP, Couvillion AM, Binnie WH, Hasegawa S, Lam LKL (1994) Citrus limonoids as inhibitors of oral carcinogenesis. Food Technol. 48:110-114
  13. Park GY, Lee SJ, Im JG (1997) Effect of green tea catechin on cytochrome xanthine oxidase activities in liver and liver damage in streptozotochin induced diabetic rats. J Korean Soc. Food Nutr. 26:901-907
  14. Park YS, Chang HG (2003) Lactic acid fermentation and biological activities of Rubus coreanus. J. Korean Soc. Agric. Chem. Biotechnol. 46(4):367-375
  15. Riccardo S, Filippo B, Ida D, Pier GP (1995) Flavonoid-DNA interaction studied with flow linear dichroism technique. J. Agric. Food Chem. 43:876-882 https://doi.org/10.1021/jf00052a006
  16. Shin KH, Lim SS, Lee SH, Seo JS, Yu CY, Park CH (1998) Vitamin content in Rosa davurical Pall. Kor J. Medicinal Crop Sci. 6:6-10