Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Plant Regeneration from Leaf Explants of Kalanchoe daigremontiana Hamet & Perrier

  • ;
  • Kim, Teh-Ryung (Department of Genetics, University of Georgia) ;
  • In, Jun-Gyo (Research Instititute of Biotechnology, Biopia Co., Ltd.) ;
  • Yang, Deok-Chun (Dept. of Oriental Medicine Material and processing, Kyung Hee Univ.) ;
  • Choi, Kwan-Sam (Department of Applied Biology, Chungnam National University)
  • Published : 2006.10.01
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Abstract

Optimum culture conditions for high frequency plant regeneration from leaf explants of Kalanchoe daigremontiana Hamet &Perrier were established. Shoot regeneration was achieved from leaf explant cultures using MS medium supplemented with indole-3-acetic acid (IAA) and thidiazuron (TDZ) or benzyladenine (BA). Percent regeneration was influenced by plant growth regulators and source of explants. MS medium supplemented with TDZ (1.0 mg/l) and IAA (0.4 mg/l) was the most effective, providing shoot regeneration for 76.7 % of ex vitro leaf explants associated with a high number of shoots per explant (9.5 mean shoots per explant), whereas 100% shoot regeneration associated with 12.4 shoots per explant occurred from in vitro leaf explants on the same medium. Clusters of shoots were multiplied and elongated on MS medium containing several concentrations of BA. MS medium supplemented with 0.25 mg/l BA was proved as the most effective shoot elongation medium. Elongated shoots (2-3 cm) were rooted at 100% on half-strength MS medium. Rooted plantlets were then transferred to potting soil. Regenerated plants were established in the soil with 90% success.

Keywords

References

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