Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Anchovy Treated with Ethanol, Citric Acid and Dietary Calcium Supplements on Calcium Metabolism in Rats

주정과 구연산 및 식이성 칼슘소재를 처리한 멸치분말이 흰쥐의 칼슘대사에 미치는 영향

  • Published : 2006.08.30
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Abstract

This study was performed to investigate the effect of ethanol and citric acid-treated anchovy, caseino-phosphopeptides (CPPs), calcium lactate, and calcium phosphate as dietary calcium supplements on calcium metabolism in rats for 5 weeks. Experimental animals were randomly assigned to five treatments with 15 heads of SD male rats (mean body wt. of 100 g) in each group. The experimental diets were as follows; dried large anchovy powder (C) as control, ethanol+citric acid group (EC), ethanol+citric acid+cpps group (ECC), calcium lactate group (CL) and calcium phosphate group (CP), which were formulated with commercial semi-purified Chow diet, while maintaining the same level of calcium in all diets (1%) groups. The weight gain of EC group was significantly higher than ECC, CL and CP groups (p<0.05), food efficiency (FER) was not different. In vitro and in vivo calcium absorption rates of ECC group treated with citric acid and CPPs were 20.4 and 28.4%, respectively, and the highest among the experimental groups (p<0.05). The blood glucose levels of CL group (105.7 mg/dL) was significantly higher than control group (98.5 mg/dL). In terms of serum lipids, total-cholesterol concentration of EC group (75.1 mg/dL) was significantly higher than CP group (65.6 mg/dL) and triglyceride concentration of CP group (33.5 mg/dL) was the lowest (p<0.05). ALP activity and 057 level were not different among experimental groups. The serum calcium concentration of control group (C) was the lowest among groups (p<0.05). The femur weight of CP group was the lowest (p<0.05) and the femur length of ECC group is the longest (P<0.05). The bone density of CP group $(0.1116\;g/cm^2)$ was the lowest while ECC group $(0.1149\;g/cm^2)$ was the highest, and the bone density was increased by added CPPs. These data demonstrated that ECC group significantly increased in vitro and in vivo calcium absorption rate, serum Ca level, and the length and bone density of femur.

본 연구는 주정과 구연산을 처리한 대멸분말과 함께 식이성 칼슘소재가 SD계 흰쥐의 칼슘대사에 미치는 영향을 조사하기 위하여 수행되었다. 실험식이는 대델분말의 칼슘함량을 고려하여 식이중의 칼슘함량이 0.1%가 되도록 semi-purified diet(AIN-diet, 1977)에 건조대별분말을 첨가한 대조군(C), 주정-구연산처 리군(EC), 주정-구연산+CPPS처리군(ECC), 젖산칼슘첨가군(CL) 및 인산칼슘침가군(CP)으로처리하여 5주동안 실험한 결과는 다음과 같다. 증체랑은 EC군이 ECC군과 일반적인 칼슘소재로 이용되는 젖산칼슘군(CL)과 인산칼슘군(CP)에 비해 유의하게 높았고(p<0.05), 식이효율(FER)은 차이가 없었다. 생체내외(in vitro 을 in vivo) 칼슘흡수율은 CPP를 처리한 ECC군이 각각 20.4%,28.4%로 실험군중 가장 높았다(p<0.05). 혈당치는 CL군(105.7 mg/dL)이 대조군(89.5 mg/dL)보다 유의하게 높았고 (p<0.05), TC농도는 EC군(75.1 mg/dL)이 CP군(65.6 mg/dL)보다 높았으며, TG농도는 CP군(33.5 mg/dL)이 통계적으로 가장 낮았다(p<0.05). ALP활성과 057농토는 실험군간 차이가 없이 CL군이 대조군보다 다소 높았다. 혈중 Ca농토는 칼슘흡수율이 가장 낮은 대조군(C)이 10.82 mg/dL로 유의하게 낮았고, EC군과 ECC군이 가장 높았다(p<0.05). 대퇴골무게는 CP군이 가장 낮았고(p<0.05),길이는ECC군이 가장 길었다(P<0.05). 골밀도는 CP군$(0.1116\;g/cm^2)$이 가장 낮았던 반면, ECC군$(0.1149\;g/cm^2)$이 가장 높았다. 이상의 결과에서 CPPs를 첨가한 ECC군이 생체내의 칼슘흡수율과 혈중 Ca농도 및 대퇴골의 길이와 밀도 등에 유의한 상승효과를 미친 것으로 나타났다. 향추 본 연구결과는 칼슘흡수율을 높이고 색택의 개선 및 관능이 향상된 기능성제품 개발의 기초자료로 활용될 수 있을 것으로 사료된다.

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References

  1. Einhorn TA, Levine B, Michel P. 1990. Nutrition and bone. Ortho Clin Nor Am 21: 43-50
  2. Ministry of Health and Welfare. 2002. '2001 National Nutrition Survey Report'
  3. Allen JH, Wood RJ. 1994. Calcium and phophorus. In Modern Nutrition in Health and Disease. Shills ME, Olson JA, Shike M, eds. 8th ed. Lea & Febiger, Pholadelphia. p 144-163
  4. Heaney RP. 1993. Nutritional factors in osteoporosis. Annu Rev Nutr 13: 287-316 https://doi.org/10.1146/annurev.nu.13.070193.001443
  5. Lee YS, Park JH, Cho CW. 1992. Effect of bovine bone powder as a dietary calcium source on mineral bioavailability in rats. Korean J Rural Living Sci 3: 17-26
  6. Chang SO. 2003. A study on the calcium bioavaility of eggshell powder in the gowing rats. Korean J Nutr 36: 684-690
  7. Kim JW, Hur JW. 2002. Improvement of functional properties of mayonnaise with egg-shell calcium and chitosan. Food Engineering Progress 6: 195-200
  8. Shin HS, Kim KH, Yoon JG. 1998. Rheological properties of cooked noodle fortified with organic acids-eggshell calcium salts. Korean J Food Sci Technol 30: 1197-1202
  9. The Fisheries Association of Korea. 1998. Korean Fisheries Yearbook. Dongyang Publishing Co., Seoul. p 354-363
  10. Lee EH, Kim SK, Cho GD. 1977. Nutritional component and health in the fishery resources of the coastal and offshore waters in Korea. Youil Publishing Co., Busan. p 43-46
  11. Lee SH, Chang SO. 1994. Comparison of the bioavailability of calcium from anchovy, tofu and nonfat dry milk (NFDM) in growing male rats. Korean J Nutr 27: 473-482
  12. Lee EH, Ahn CB, Oh KS, Lee TH, Cha YJ, Lee KW. 1986. Studies on the processing of low salt fermented sea foods: 9. Processing conditions of low salt fermented small shrimp and its flavor components. J Korean Fish Soc 19: 459-468
  13. Lee EH, Kim SK, Jeon JK, Cha YJ, Chung SH. 1981. The taste compounds in boiled-dried anchovy. Bull Korean Fish Soc 14: 194-200
  14. Lee EH, Park YH. 1971. Degradation of acid soluble nucleotides and their related compounds in seafoods during processing and storage. Bull Korean Fish Soc 4: 31-41
  15. Lee EH, Kim JS, Ahn CB, Park HY, Jee SK, Joo DS, Lee SW, Lim CW, Kim IH. 1989. The effect of taipet-F and bactokil on retarding lipid oxidation in boiled-dried anchovy. J Korean Soc Food Nutr 18: 181-188
  16. Lee EH, Cha YJ. 1985. Studies on the processing of low salt fermented sea foods 5. Processing conditions of low salt fermented anchovy and yellow corvenia. J Korean Fish Soc 18: 206-213
  17. Jeong BY, Seo HJ, Moon SK, Pyeun JH. 1995. Effect of deoxygenizer on the suppression of lipid deterioration of boiled and dried-anchovy, Engraulis japonica. 1. Changes in lipid class composition. J Korean Fish Soc 28: 770-778
  18. Jo JH, Jang HJ, Cho SM, Lee YB. 2005. Effects of ethanol and organic acids on color, fishy odor and in vitro absorption rate of calcium of dried large anchovy. J Korean Soc Food Sci Nutr 34: 1471-1476 https://doi.org/10.3746/jkfn.2005.34.9.1471
  19. American Institute of Nutrition. 1977. Report of the American Institute of Nutrition ad hoc Committee on Standards for Nutritional Studies. J Nutr 107: 1340-1348 https://doi.org/10.1093/jn/107.7.1340
  20. Miller DD, Schricker BR, Rasmussen RR, Campen DV. 1981. An in vitro method for estimation of iron availability from meals. Am J Clin Nutr 34: 2248-2256 https://doi.org/10.1093/ajcn/34.10.2248
  21. Kim YM, Yoon GA, Hwang HJ, Chi GY, Son BY, Bae SY, Kim IY, Chung JY. 2004. Effect of bluefin tuna bone on calcium metabolism of the rat. J Korean Soc Food Sci Nutr 33: 101-106 https://doi.org/10.3746/jkfn.2004.33.1.101
  22. Pointillart A, Gueguen L. 1989. Absence d'effet de l'incorporation d'un phosphopeptide du lait sur l'utilisation du calcium et du phosphore chez le jeune porc. Reprod Nutr Dev 29: 477-486 https://doi.org/10.1051/rnd:19890409
  23. Hiroshi T, Toshiyuki G, Yoko Y, Tamotsu K. 1995. Calcium and phosphorus availability from casein phosphopeptides in male growing rats. Nutrition Research 15: 1657-1667 https://doi.org/10.1016/0271-5317(95)02036-7
  24. Sato R, Noguchi T, Naito H. 1986. Casein phosphopeptide (CPP) enhances calcium absorption from the ligated segment of rat small intestine. J Nutr Sci Vitaminol 32: 67-76 https://doi.org/10.3177/jnsv.32.67
  25. Erba D, Ciappellano S, Testolin G. 2002. Effect of the ratio of casein phosphopeptides to calcium (w/w) on passive calcium transport in the distal small intestine of rats. Nutrition 18: 743-746 https://doi.org/10.1016/S0899-9007(02)00829-8
  26. Kim GH, Jeon YJ, Byun HG, Lee YS, Lee EH, Kim SK. 1998. Effect of calcium compounds from oyster shell bouind fish skin gelatin peptide in calcium deficient rats. J Korean Fish Soc 31: 149-159
  27. Kitts DD, Yuan YV. 1992. Casein phosphopeptides and calcium bioavailability. Trends in Food Sci Technol 3: 31-35 https://doi.org/10.1016/0924-2244(92)90113-B
  28. Lee CK, Choi JS, Jeon YJ, Byun HG, Kim SK. 1997. The properties of natural hydroxyapatite isolated from tuna bone. Bull Korean Fish Soc 30: 652-659
  29. Aloia JF, Cohr SH, Vaswani A, Yeh JK, Yuen K, Ellis K. 1985. Risk factors for postmenopausal osteoporosis. Am J Med 78: 95-100 https://doi.org/10.1016/0002-9343(85)90468-1
  30. Han CK. 2005. Effect of pluskids-calcium formulation on growth and development in growing rats. Korea Food Research Institute Report (I01658-05006). p 21
  31. Yoon GA, Hwang HJ. 2005. Effect of dietary protein and calcium levels on calcium metabolism of the rat. J Korean Soc Food Sci Nutr 34: 176-180 https://doi.org/10.3746/jkfn.2005.34.2.176
  32. Moon SJ, Kim JH, Lim SK. 1996. Investigation of risk of low serum 25-hydroxyvitamin D lever in Korean menopausal women. Korean J Nutrition 29: 981-990

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