한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Shigella sonnei ATCC 29930의 아치사 가열 후 소금 농도에 따른 회복 정도 비교

Comparison of Recovery Levels of Shigella sonnei ATCC 29930 Treated at Different NaCl Concentrations after Sublethal Heating

  • 정혜진 (중앙대학교 식품공학과) ;
  • 박성희 (중앙대학교 식품공학과) ;
  • 송은섭 (인하대학교 의과대학 산부인과학교실) ;
  • 박성수 (제주한라대학 제주향토식품연구소) ;
  • 김근성 (중앙대학교 식품공학과)
  • Jung, Hye-Jin (Department of Food Science and Technology, Chung-Ang University) ;
  • Park, Sung-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Song, Eun-Seop (Department of Obstetrics and Gynecology, College of Medicine, Inha University) ;
  • Park, Sung-Soo (Cheju Traditional Food Institute, Cheju Halla College) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
  • 발행 : 2006.10.01
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초록

본 연구에서는 우리 나라 세균성 이질을 유발하는 주요 원인 균인 S. sonnei를 대상으로 불충분한 열처리와 NaCl 처리에 따른 각각의 생존균수 변화와 불충분한 열처리 후 다양한 NaCl 농도 (0, 2, 4, 6%) 하에서 sublethal injured cell의 회복정도를 조사하였다. 그 결과, 불충분한 열처리 정도가 증가함에 따라 생존균수가 유의적인 감소를 나타내었으나, NaCl 처리시 NaCl 농도가 6% 까지 증가함에 따라서 생존균수의 변화가 거의 없었다. 그리고 $55^{\circ}C$에서 30분 동안 열처리후 2% TSAS 배지에서 7.58 log CFU/mL, 4% TSAS 배지에서 7.83 log CFU/mL, 그리고 6% TSAS 배지에서 7.93 log CFU/mL 만큼 각각 생성된 colony 숫자가 감소 한 것으로 조사되었고, $60^{\circ}C$에서 30분동안 열처리후 2% TSAS 배지에서 6.71 log CFU/mL, 4% TSAS 배지에서 6.73 log CFU/mL, 그리고 6% TSAS 배지에서 6.73 log CFU/mL 만큼 각각 생성된 colony 숫자가 감소한 것으로 조사되었다. 그리고 동일한 온도에서 sublethal heating후 노출된 NaCl 농도가 증가함에 따라서 D-value가 감소하는 경향을 나타내었다. 이와 같은 결과는 불충분한 열처리 과정에 의하여 생성된 sublethal injured cell들이 열처리후 회복되는 과정에서 배지의 NaCl 농도가 증가함에 따라서 회복정도가 감소하였음을 의미한다.

The viability of Shigella sonnei, a significant cause of gastroenteritis in Korea, on TSA plates was determined after sublethal heating treatments and NaCl treatments. In addition, recovery levels of sublethally injured cells on TSA plates containing different concentrations of NaCl (TSAS) were investigated. The viability decreased significantly with increasing degree of sublethal heating treatments, but increases in NaCI treatment concentration from 0 to 6% had little effect on the viability. After being sublethally treated at $55^{\circ}C$ for 30 min, bacterial populations were reduced by 7.58, 7.83 and 7.93 log CFU/mL on 2, 4, and 6% TSAS, respectively. After being sublethally treated at $60^{\circ}C$ for 30 min, bacterial populations were reduced by 6.71, 6.73, and 6.73 log CFU/mL on 2, 4 and 6% TSAS, respectively. Decimal reduction times (D-values) decreased with increasing NaCl treatment concentrations after sublethal heating at 55 or $60^{\circ}C$. These data imply that the S. sonnei cells sublethally injured by insufficient heating processes had a lower recovery rate with increasing NaCl concentrations in the recovery media.

키워드

참고문헌

  1. Mohle-Boetani JC, Stapleton M, Finger R, Bean NH, Poundstone J, Blake PA, Griffin PM. Communitywide shigellosis: control of an outbreak and risk factors in chi Id day-care centers. Am. J. Public Health 85: 812-816 (1995) https://doi.org/10.2105/AJPH.85.6.812
  2. Terajima J, Tamura K, Hirose K, Izumiya H, Miyahara M, Konuma H, Watanabe H. A multi-prefectural outbreak of Shigella sonnei infections associated with eating oysters in Japan. Microbiol. Immunol. 48: 49-52 (2004) https://doi.org/10.1111/j.1348-0421.2004.tb03486.x
  3. Gupta A, Polyak CS, Bishop RD, Sobel J, Mintz ED. Laboratory-confirmed shigellosis in the United States, 1989-2002: epidemiologic trends and patterns. Clin. Infect. Dis. 38: 1372-1377 (2004) https://doi.org/10.1086/386326
  4. Kim YB, Moon JY, Lee BK. Antibiotic resistance and genetic analysis of Shigella sonnei strains isolated in South Korea and Japan. J. Bacteriol. Virol. 34: 93-102 (2005)
  5. Blackburn CW, Curtis LM, Humpheson L, Billon C, McClure PJ. Development of thermal inactivation models for Salmonella enteritidis and Escherichia coli O157:H7 with temperature, pH and NaCI as controlling factors. Int. J. Food Microbiol. 38: 31-44 (1997) https://doi.org/10.1016/S0168-1605(97)00085-8
  6. Li X, Sheldon BW, Ball HR. Thermal resistance of Salmonella enterica serotypes, Listeria monocytogenes, and Staphylococcus aureus in high solids liquid egg mixes. J. Food Prot. 68: 703-710 (2005) https://doi.org/10.4315/0362-028X-68.4.703
  7. Bunning VK, Crawford RG, Tierney JT, Peeler JT. Thermotolerance of Listeria monocytogenes and Salmonella typhimurium after sublethal heat shock. Appl. Environ. Microbiol. 56: 3216-3219 (1990)
  8. Kang DH, Siragusa GR. Agar underlay method for recovery of sublethally heat-injured bacteria. Appl. Environ. Microbial. 65: 5334-5337 (1999)
  9. Taormina PJ, Beuchat LR. Survival and heat resistance of Listeria monocytogenes after exposure to alkali and chlorine. Appl. Environ. Microbial. 67: 2555-2563 (2001) https://doi.org/10.1128/AEM.67.6.2555-2563.2001
  10. Iandolo JJ, Ordal ZJ. Repair of thermal injury of Staphylococcus aureus. J. Bacterial. 91: 134-142 (1966)
  11. Smolka LR, Nelson FE, Kelley LM. Interaction of pH and NaCI on enumeration of heat-stressed Staphylococcus aureus. Appl. Microbial. 27: 443-447 (1974)
  12. Hernandez FJ, Goyache J, Orden JA, Blanco JL, Domenech A, Suarez G, Gomez-Lucia E. Repair and enterotoxin synthesis by Staphylococcus aureus after thermal shock. Appl. Environ. Microbial. 59: 1515-1519 (1993)
  13. Clavero MR, Beuchat LR. Survival of Escherichia coli O157:H7 in broth and processed salami as influenced by pH, water activity, and temperature and suitability of media for its recovery. Appl. Environ. Microbial. 62: 2735-2740 (1996)
  14. Juneja VK, Klein PG, Marmer BS. Heat shock and thermotolerance of Escherichia coli O157:H7 in a model beef gravy system and ground beef. J. Appl. Microbial. 84: 677-684 (1998) https://doi.org/10.1046/j.1365-2672.1998.00396.x
  15. Duffy G, Riordan DCR, Sheridan JJ, Eblen BS, Whiting RC, Blair IS, McDowell DA. Differences in thermotolerance of Escherichia coli 0157:H7 strains in a salami matrix. Food Microbial. 16: 83-91 (1999) https://doi.org/10.1006/fmic.1998.0214
  16. Ahmed NM, Conner DE, Huffman DL. Heat-resistance of Escherichia coli O157:H7 in meat and poultry as affected by product composition. J. Food Sci. 60: 606-610 (1995) https://doi.org/10.1111/j.1365-2621.1995.tb09838.x
  17. Zaika LL, Phillips JG. Model for the combined effects of temperature, pH and sodium chloride concentration on survival of Shigella flexneri strain 5348 under aerobic conditions. lnt. J. Food Microbial. 101: 179-187 (2005) https://doi.org/10.1016/j.ijfoodmicro.2004.11.004
  18. Zaika LL. The effect of NaCI on survival of Shigella flexneri in broth as affected by temperature and pH. J. Food Prot. 65: 774-779 (2002) https://doi.org/10.4315/0362-028X-65.5.774
  19. Novak JS, Juneja VK. Detection of heat injury in Listeria monocytogenes Scott A. J. Food Prot. 64: 1739-1143 (2001) https://doi.org/10.4315/0362-028X-64.11.1739
  20. Juneja VK, Eblen BS. Heat inactivation of Salmonella typhimurium DT104 in beef as affected by fat content. Lett. Appl. Microhiol. 30: 461-467 (2000) https://doi.org/10.1046/j.1472-765x.2000.00755.x