Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Monitoring and Analysis of Genetically Modified Ingredients of Imported Foods by PCR

PCR에 의한 수입식품의 유전자재조합 원료 분석 및 모니터링

  • 김희연 (경인지방식품의약품안전청 시험분석팀) ;
  • 박용춘 (경인지방식품의약품안전청 시험분석팀) ;
  • 노혜림 (경인지방식품의약품안전청 시험분석팀) ;
  • 조준일 (경인지방식품의약품안전청 시험분석팀) ;
  • 김은정 (경인지방식품의약품안전청 시험분석팀) ;
  • 남혜선 (경인지방식품의약품안전청 시험분석팀) ;
  • 이진경 (경인지방식품의약품안전청 시험분석팀) ;
  • 이진하 (경인지방식품의약품안전청 시험분석팀) ;
  • 강윤숙 (식품의약품안전청 식품미생물팀) ;
  • 이종옥 (식품의약품안전청 식품오염물질팀)
  • Published : 2006.10.01
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Abstract

Genetically modified (GM) ingredients found in imported raw materials and processed foods were monitored in the province Gyeongin in Korea. The analysis was performed according to "Testing methods for genetically modified foods of food standards and specifications" established in Korea. We received 120 items from the Gyeongin Regional KFDA. Only two of the 120 items analyzed in the samples, were contaminated with GM ingredients. However, we could not analyze the internal standard gene from 12 processed foods. We found that the extracted total DNA of the above 12 samples were extracted and found to be degraded. The total DNA contained a very small fragment of less than 300 base pair. Therefore, it seems that the total DNA is not large enough to serve as the template DNA for PCR analysis.

2004년 9월부터 12월까지 경인지역으로 수입되는 원료농산물 및 가공식품에 대하여 유전자재조합성분 함유여부 및 유전자재조합식품 표시제 이행실태를 확인하기 위하여 모니터링을 실시하였다. 분석방법은 유전자재조합식품 검사지침에 따랐으며, 콩류가공품(원료콩 포함) 64건, 옥수수 가공품(원료 옥수수 포함) 52건, 콩 및 옥수수 함유식품 4건 등 총 120 건을 분석대상으로 하였다. 분석결과 콩을 원료로 하는 콩류가공품 1건, 콩 및 옥수수 혼합 가공식품 1건 등 총 2건에서 유전자재조합 콩인 round-ready soybean 성분을 확인 할 수 있었다. 그러나 유전자재조합 옥수수 (Bt11, GA21, T25, E176, Mon810)를 포함하는 가공식품은 확인되지 않았다. 그리고 콩 가공품 3건, 옥수수가공품 8건, 콩 및 옥수수가공품 1건 등 총 12건에서는 내재성유전자를 확인할 수 없어 검사불능으로 판정되었다. 유전자재조합성분이 확인된 시료 2건은 구분유통증명서를 구비하고 있었으며, 검사불능 시료의 경우 대부분 옥수수전분을 포함하는 제품으로 확인되었고, 추출된 유전자를 양상을 분석한 결과 크기가 300 bp 이하로 절단되어 PCR법을 이용한 분석 시 주형유전자로 사용하기에는 충분 하지 않은 것으로 판단된다.

Keywords

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