Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Hypoglycemic Effect of the Giant Embryonic Rice Supplementation on Streptozotocin-induced Diabetic Rats

거대배아미 식이에 의한 Streptozotocin 유발 당뇨 흰쥐의 혈당강하 효과

  • Lee, Youn-Ri (Department of Food Science and Nutrition, Kyunpook National University) ;
  • Nam, Seok-Hyun (Department of Biological Science, Ajou University) ;
  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyunpook National University)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the influence of giant embryo feeding on hypoglycemia in streptozotocin-induced diabetic rats. Streptozotocin -induced diabetic rats were fed four kinds of experimental diets such as corn starch diet as a control, polished rice diet, brown rice diet and giant embeyonic rice diet for 6 weeks. The body weight gain diabetic of control was signaficantly suppressed compared to that of the normal. The supplementation of giant embryo increased the surivival rate. Feeding of giant embryo appeared to have ameliorating effects on diabetic symptoms including features such as polyphasia, polyuria. Giant embryo shortened gastrointestinal transit time and increased total fecal weight, total fecal dry weight and fecal water content compared with diabetic control. Giant embryo showed fasting blood glucose lowering effect compared with diabetic control. The disaccharidase activities in proximal part of intestine such as maltase sucrase and lactase in giant embryo feeding groups were lower than diabetic control. The results of this study show that giant embryo supplementation may have a beneficial Veffect on the hypoglycemia may be useful in the diet therapy for diabetic.

췌장에 ${\beta}-cell$에만 특이적으로 작용하고 다른 기관에는 영향을 주지 않는 것으로 보고되어 있는 streptozotocin을 흰쥐에게 투여하여 당뇨병을 유발시킨 다음 백미, 현미, 거대배아미를 6주간 급여한 후 혈당강화 효과를 알아보았다. 실험기간동안 모든 당뇨군들의 체중증가는 정상군에 비해서는 유의적으로 낮았다. 당뇨대조군에 비해서는 거대배아마를 급여한 군에서 체중감소현상이 억제되었다. 장기중 맹장의 경우 2배 이상의 비대현상이 나타났고 정상쥐에 비해 당뇨쥐가 수분섭취량, 소변배설량, 혈당이 유의적으로 높게 나타났는데 당뇨병의 주요 증세가 다갈(polydipsia), 다뇨(polyuria), 다식(polyphagia), 고혈당인점에서 당뇨군에 비해서 가대배아미는 당뇨증세가 어느 정도 호전효과가 있음을 보여주고 있다. 식이섭취량은 정상군에 비해 당뇨실험군들이 높게 나타났으며 수분섭취량은 정상군에 비해 당뇨대조군이 수분 섭취량증가가 나타났다. 당뇨실험군 내에서는 당뇨대조군에 비해 가대배아미군이 수분섭취량이 감소하였다. 뇨 배설량은 정상군에 비해 당뇨대조군이 유의적으로 높게 나타났으며 당뇨대조군에 비해서 거대배아미군이 감소하였다. 장 기능 조절로서 장통과 시간과 분변고형물의 양에는 역의 상관관계로 식이섬유소의 함량이 높은 쌀 품종인 거대배아미는 장통과 시간이 짧으며 분변고형물의 양을 유의적으로 증가시켰으며 변의 수분보유량도 정상군에 비해 당뇨실험군들중 거대배아미군 전반적으로 높았다. 위 결과에서 거대배아미 급여가 당뇨동물의 공복시 혈당 수준을 유의적으로 낮추는데 효과가 있었으며 이당류 분해 효소 활성의 경우 정상군에 비해서 당뇨군이 lactase, maltase, sucrase 활성이 현저하게 증가 되었지만 당뇨실험군중에는 거대배아미가 소장점막부분의 lactase, maltase, sucrase의 활성을 저해시킴으로서 혈당상승을 억제시키는 것으로 볼 수 있었다. 이상의 결과로 백미, 현미에 비해서 거대배아미를 급여한 당뇨동물에서 혈당을 억제시키는 효과를 보았다.

Keywords

References

  1. Seo JS, Lee KS, Jang JH. Effect of dietary supplementation of ${\beta}$-carotene on lipid peroxide level and antioxidative vitamins of diabetic rats. J. Korean Soc. Food Sci. Nutr. 33: 72-77 (2004) https://doi.org/10.3746/jkfn.2004.33.1.072
  2. Ha AW, Kim HM. The study of lipid-peroxidation, antioxidant enzymes and the antioxidant vitamin in NIDDM patients with microvascular-diabetic complications. Korean J. Nutr. 31: 1139-1150 (1999)
  3. Lee DM, Hoffma WH, Carl GF, Cornwell PE. Lipid peroxidation vitamin prior to during and after correction of diabetes ketoacidosis. J. Korean Diabetes Complications. 16: 294-300 (1999)
  4. Son HY, Hotta N, Sukamoto N, Natenoka S, Ohishi N, Yagi N. Lipoprotein(a) and diabetes mellitus. J. Korean Diabetes Assoc. 16: 275-280(1992)
  5. Igarshi K, Ohmune M. Effects of isohamneth rhamnetin and quercetin on the concentation of cholesterol and lipoperoxide in the serum and liver and on the blood and liver antioxidative enzyme activities or rats. Biosci. Biotechnol. Biochem. 59: 595-601 (1995) https://doi.org/10.1271/bbb.59.595
  6. Vahouny GV, Roy T, Gallo LL, Story J. Kritchevsky D, Cassidy M, Grund BM. Dietary fiber and lymphatic absorption of cholesterol in the rat. Am. J din. Nutr. 31: 208-212 (1978)
  7. Arky RA. National management of diabetic. pp. 539-566. In: Diabetes Mellitus: Theory and practice. 3rd ed.. Medical Examination Publishing Co., NY, USA (1983)
  8. Crapo PA, Insel J, Sperling M, and Kolterman OG. Comparison of serum glucose, insulin, and glucagon responses to different types of complex carbohydrate in noninsulin-dependent diabetic patients. Am. J. Clin. Nutr. 34: 184-190 (1981) https://doi.org/10.1093/ajcn/34.2.184
  9. Jenkins DJA, Wolever TMS. Slow release carbohydrate improves second meal tolerance. Am. J. Clin. Nutr. 35: 1339-1346 (1982) https://doi.org/10.1093/ajcn/35.6.1339
  10. Gordon DT. The importance of total dietary tiber in human nutrition and health. Korean J. Nutr. 25: 75-82 (1992)
  11. Kim MJ, Lee SS. The effect of dietary fiber on the seurm lipid level and bowel function in rats. Korean J. Nutr. 28: 23-32 (1995)
  12. Schweizer TH, Wurseh P. The Physiological and nutritional importance of dietary fiber. Experimentia 47: 181-186 (1987)
  13. Hwang SH, and Park YJ. Assessment of dietary tiber intake in Korean college students. J. Korean Soc. Food Nutr. 25: 205-213 (1996)
  14. Rhie SG, Park YJ. The comparison of health status and dietarv health practice with or without hypertension of middle-aged rural adults in Kyungi province. Korean J. Rural Living Sci. 8: 131-143 (1997)
  15. Na HJ, Kim YN. The prevalence of constipation and dietary fiber intake of 3rd grade high school girls. Korean J. Nutr. 33: 675-683 (2000)
  16. Baek JW, Koo BK, Kim KJ, Lee YK, Lee HS. Nutritional status in the ling-live elderly people in Kyungpook Sung-Ju area (I): Estimation of nutritions intake. Korean J. Nutr. 33: 438-453 (2000)
  17. Lee SH, Park HJ, Cho SY, Han GJ, Chun, HK, Hwang, HG Choi HC. Supplementary effect of the high dietary fiber rice on lipid metabolism in diabetic KK rice. Korean J. Nutr. 37: 81-87 (2004)
  18. Lee YT. Preparation and hypoglycemic effect of reconstituted grain added with selected medicinal herb extract. Korean J. Food Sci. Technol. 35: 527 -531 (2003)
  19. Sato H, Omura T. New endosperm mutations induced by chemical mutagens in rice. Jpn. J. Breed. 31: 316-326 (1981) https://doi.org/10.1270/jsbbs1951.31.316
  20. Kang MY, Lee YR, Nam SH. Some Physical properties of starch granules lrom giant embryonic rice endospern. J. Korean. Soc. Agric. Chem. Biot. 46: 117-122 (2003)
  21. Kang MY, Lee YR, Nam SH. Antioxidative and Antimutagenic activity of ethanolic extracts from giant embrynoic rices. J. Korean. Soc. Appl. Biol. Chem. 47: 61-66 (2004)
  22. Kang MY, Kim SY, Koh HJ, Jin JH, Nam SH. Antioxidative activity of germinated specialty rices, Korean J. Food Sci. Technol. 36: 624-630 (2004)
  23. Lazarus SS, Shapio SH, Streptpzotocin-induced diabetes and iselt cell alteration in rabbits. Diabetes 21: 129-137 (1972) https://doi.org/10.2337/diab.21.3.129
  24. Dahlquist A. Assay of intestine disaccharides scand. J. Clin. Lab. Invest. 44: 169-172 (1984) https://doi.org/10.3109/00365518409161400
  25. Bradford MM. A rapid and sensitivies mathod for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  26. Best JD. Diabetic dyslipidaemia-current treatement recommendaions. Drugs 59: 1101-1111 (2000) https://doi.org/10.2165/00003495-200059050-00006
  27. Daniel DG, Saari C. Don WS, Judith MO. Diabetes increase excretion of urinary malonaldehyde conjugates in rats. Lipid 28: 663-666 ( 1993) https://doi.org/10.1007/BF02536063
  28. Zachania M. Effects of brown rice and soybean diatery fiber on the control of glucose and lipid metabolism in diabetic rats. Am. J. Clin Nutr. 38: 388-393 (1983) https://doi.org/10.1093/ajcn/38.3.388
  29. Lee WJ, Kim SS. Preparation of brown rice. Korean J. Food Sci. Technol. 30: 101-106 (1998)
  30. Shon KH, Kim SH, Chio JW. Pretreatement with nicotinamide to prevent pancreatic enzymes changes by streptozotocin in rats. J. Korean Soc. Food Nutr. 21: 117-123 (1992)
  31. Southgate DAT. The relationship between composition and properties of dietary fiber and physiological effects In: Dietary fiber basic and clinical aspects plenum. 35-48 (1986)
  32. Kiehm TG. Beneficial effects of a high carbohydrate fiber diet on hyperglycermic diabetic men. Am. J. Clin. Nutr. 29: 895-899 (1976) https://doi.org/10.1093/ajcn/29.8.895
  33. Anderson JW, Ward K. High carbohydrate tiber diet on hyperglycermic diabetic men. Am. J. Clin. Nutr. 32: 2312-2321 (1979) https://doi.org/10.1093/ajcn/32.11.2312