Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

In vitro Screening of Medicinal Plants with Estrogen Receptor Modulation Activity

생약의 여성호르몬 수용체 조절 활성 검색

  • 이창민 (성균관대학교 약학대학) ;
  • 강세찬 (성균관대학교 약학대학) ;
  • 오좌섭 (성균관대학교 약학대학) ;
  • 최한 (성균관대학교 약학대학) ;
  • 이설매 (성균관대학교 약학대학) ;
  • 이재현 (성균관대학교 약학대학) ;
  • 이미현 (성균관대학교 약학대학) ;
  • 정의수 (성균관대학교 약학대학) ;
  • 곽종환 (성균관대학교 약학대학) ;
  • 지옥표 (성균관대학교 약학대학)
  • Published : 2006.03.30
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Abstract

Yeast based estrogenicity assay is the simplest and useful for the assay and the discovery of novel estrogenic substances in natural specimens, The estrogen receptor(ER) modulation activity of 50% EtOH extracts of 101 traditional medicinal herbs was assessed using a recombinant yeast assay system with both a human estrogen receptor expression plasmid and a receptor plasmid. Among them, 14 species proved to be active. Pureariae Flos (flower of Puerraria thunbergiana BENTH.) had the highest estrogenic relative potency$(7.75{\times}10^{-3})$ $(EC_{50}=9.39\;{\mu}g/ml)$. The $EC_{50}$ value of $17{\beta}-estradiol$ used as the positive control was $0.073\;{\mu}g/ml)$ (Relative Potency=1.00). There results demonstrated that some of the traditional medical herb may be useful in the therapy of estrogen replacement.

Keywords

References

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