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Whitening Effect of Mycelial Culture Broth of Paecilomyces japonica in the Mixture of Cucumber and Crape Extracts

포도와 오이즙액을 이용한 동충하초(Paecilomyces japonica) 균사체 배양액의 미백효과

  • Lee, Young-Hoon (Department of Microbiological Engineering, Jinju National University) ;
  • Park, Sang-Sang (Department of Microbiological Engineering, Jinju National University) ;
  • Lee, Sang-Won (Department of Microbiological Engineering, Jinju National University) ;
  • Lee, Sung-Ho (Department of Biology, Gyeongsang National University) ;
  • Park, Ki-Hoon (Department of Applied Life Science, Gyeongsang National University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, Silla University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, Jinju National University)
  • 이영훈 (진주산업대학교 미생물공학과) ;
  • 박상상 (진주산업대학교 미생물공학과) ;
  • 이상원 (진주산업대학교 미생물공학과) ;
  • 이성호 (경상대학교 생물학과) ;
  • 박기훈 (경상대학교 응용생명과학부) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Published : 2006.08.30

Abstract

This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mixture of cucumber and grape extracts. In the inhibition test of melanin biosynthesis of melanoma cell, B16BL6, the culture broth of P. japonica more then $50\;{\mu}l/ml$ (5%) concentration inhibited the melanin biosynthesis of the cell entirely without cytotoxicity. More then 10 days incubation of P. japonica in the mixture was required to have the inhibition activity. In vitro inhibition test of melanin biosynthesis of the culture broth of P. japonica was investigated in the concentration dependent manner of 10% to 50%. 30% concentration of the culture broth inhibited completely tyrosinase activity. In the cytotoxicity test, cucumber and grape extract itself has a strong cytotoxicity to the melanoma cell, B16Bl6. The value of $IC_{50}$ of the cucumber and grape extracts against the melanoma cells was 5% concentration. However, the culture broth of P. japonica incubated in the cucumber and grape extracts did not show the cytotoxcity up to 20% against melanoma cell, B16BL6. Therefore, we concluded that the culture broth of P. japonica in the mixture of cucumber and grape extracts can be used as a whitening cosmetic resource.

포도와 오이즙액에 동충하초 균사체 배양액의 미백화장품 원료로서의 이용가능성을 조사하였다. B16BL6 mouse melanoma cell에 동충하초 균사체 배양액을 배양기간 및 농도별로 처리했을 때, 15일 동안의 배양조건과 $50\;{\mu}l/ml$ (5%)의 농도로 처리하였을 때 멜라닌 생성억제 효과가 가장 높은 것을 확인하였다. In vitro tyrosinase 활성억제효과에서는 포도와 오이즙액에 동충하초 균사체 배양액을 10% 농도로 첨가시 50%의 멜라닌 생성 억제율을 보였으며, 30% 농도에서는 100% 억제를 확인하였다. 결과적으로 포도와 오이즙액에 동충하초 균사체 배양액은 멜라닌 생성의 주 효소인 tyrosinase의 작용을 억제함으로써 미백 효과를 나타내는 것을 확인하였다. 또한 세포독성실험에서 포도, 오이즙액과 그 즙액에 동충하초 균사체 배양액을 각각의 농도로 B16BL6 mouse melanoma cell에 처리 한 결과 포도, 오이즙액의 5% 처리 시에는 50% 사멸, 9% 이상에서는 100% 세포가 사멸하는 것을 확인 할 수 있었다. 그러나 포도, 오이즙액에 동충하초 균사체를 배양한 배양액은 20% 까지 처리 시에도 세포독성이 나타나지 않는 것을 확인 하였다. 따라서 포도와 오이즙액에 동충하초 균사체를 배양한 배양액은 세포 독성이 없으며, 미백 효과가 뛰어난 것으로 미백화장품 원료로서 사용가능함을 확인하였다.

Keywords

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