생명과학회지 (Journal of Life Science)

  • 제16권5호
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  • Pages.740-745
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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ISSR을 이용한 음나무속 분류군의 유전적 다양성과 관련성 비교

Comparison of Genetic Diversity and Relationships of Genus Kalopanax Using ISSR Markers

  • 허만규 (동의대학교 분자생물학과)
  • Huh, Man-Kyu (Department Molecular Biology, Dong-eui University)
  • 발행 : 2006.08.30
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초록

ISSR 마크로 한국내 자생하는 음나무속 4분류군(음나무, 가시없는 음나무, 털음나무, 가는잎음나무)에 대해 유전적 다양성과 계통관계를 조사하였다. 64개의 재현성 높은 ISSR 밴드가 생성되었다. 음나무속의 각 개체별 분석에서 41개 밴드(64.1%)가 다형성을 나타내었다. 네 분류군을 통합하였을 때 그룹내 다양도는 0.115였고 그룹간 다양도는 0.467이였다. 종내 유전자 흐름(Nm)의 측정결과 음나무의 Nm값은 털음나무, 가는잎음나무에 비해 낮았다. 이는 지리적 거리에 따른 생식적 격리가 이 종의 집단구조를 형성하고 있다고 판단된다. 계통도 분석에서 ISSR 마크로 속수준의 네 분류군뿐만 아니라 집단까지도 잘 분리되어 본 연구에 사용한 마크가 분류에 효과적임이 규명되었다.

Inter simple sequence repeat (ISSR) markers were performed in order to analyse the phylogenetic relationships of four taxa of Castor-aralia (Kalopanax pictus): K. pictus, K. pictus var. magnificus, K. pictus var. maximowiczii, and thornless K. pictus. The 11 primers were produced 64 reproducible ISSR bands. Analysis of ISSR from individual plants of Korean K. pictus resulted in 41 polymorphic bands with 64.1%. When species were grouped by four taxa, within group diversity was 0.115 $(H_S)$, while among group diversity was 0.467 $(G_{ST})$ on a per locus basis. The estimated gene flow (Nm) for K. pictus var. maximowiczii and K. pictus var. magnificus were very higher than K. pictus. It is suggested that the isolation of geographical distance and reproductive isolation among K. pictus populations may have played roles in shaping the population structure of this species. In phenetic tree, ISSR markers are very effective in classifying natural populations as well as taxon levels of genus Kalopanax in Korea.

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피인용 문헌

  1. Genetic diversity of Kalopanax pictus populations in Korea based on the nrDNA ITS sequence vol.39, pp.1, 2012, https://doi.org/10.5010/JPB.2012.39.1.075
  2. Discrimination of Kalopanax pictus from its varieties and other ‘Kalopanacis Cortex’ plants by multiplex polymerase chain reaction (PCR) vol.33, pp.6, 2011, https://doi.org/10.1007/s13258-011-0061-x