The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Preservation of Fungi in Liquid Nitrogen Using Polypropylene Straws

폴리프로필렌 스트로를 이용한 곰팡이의 액체질소 보존

  • Jeon, Young-Ah (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology) ;
  • Shin, Myung-Sook (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology) ;
  • Kim, Hyo-Jin (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology) ;
  • Kim, Dae-Ho (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology) ;
  • Go, Seung-Joo (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology)
  • 전영아 (농업생명공학연구원 한국농업미생물자원센터(KACC)) ;
  • 신명숙 (농업생명공학연구원 한국농업미생물자원센터(KACC)) ;
  • 김효진 (농업생명공학연구원 한국농업미생물자원센터(KACC)) ;
  • 김대호 (농업생명공학연구원 한국농업미생물자원센터(KACC)) ;
  • 고승주 (농업생명공학연구원 한국농업미생물자원센터(KACC)) ;
  • 홍승범 (농업생명공학연구원 한국농업미생물자원센터(KACC))
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Liquid nitrogen storage is the most effective way to preserve many fungi including what cannot be lyophilized. The use of polypropylene straws instead of cryotubes has many advantages in economy, safety, convenience, and space-saving. We, Korean Agricultural Culture Collection (KACC), established the fungal preservation methods in liquid nitrogen using polypropylene drinking straws and introduced the methods in detail.

액체질소 보존법은 동결 건조 보존이 불가능한 것을 포함한 곰팡이를 가장 효과적으로 보존할 수 있는 방법 중의 하나로서, 유전적인 변화와 오염을 방지하고 장기보존이 가능한 이점을 가진다. 크료튜브 대신 액체질소 보존에 사용할 수 있는 폴리프로필렌 스트로는 경제성, 안전성, 편리성 그리고 공간 이용성 등의 이점을 가진다. 이에 한국농업미생물자원센터(KACC)에서는 폴리프로필렌 스트로를 이용한 곰팡이의 액체질소 보존법을 정립하였으며, 이를 상세하게 소개하였다.

Keywords

References

  1. Butterfield, W., Jong, S. C. and Alexander, M. J. 1974. Preservation of living fungi pathogenic for man and animals. Can. J. Microbiol. 20: 1665-1673 https://doi.org/10.1139/m74-258
  2. Challen, M. P. and Elliott, T. J. 1986. Polypropylene straw ampoules for the storage in microorganisms in liquid nitrogen. J. Microbiol. Methods 5: 11-23 https://doi.org/10.1016/0167-7012(86)90019-9
  3. Dietz, A. 1975. Nitrogen preservation of stock cultures of unicellular and filamentous microorganisms. Pp 22-26. In: Rinfret, A. P. and La Salle, A. B. Eds. Round table conference on cryogenic preservation of cell cultures. National Academy of Science, Washington DC
  4. Elliot, T. J. 1976. Alternative ampoule for storing fungal cultures in liquid nitrogen. Trans. Brit. Mycol. Soc. 67: 545-546 https://doi.org/10.1016/S0007-1536(76)80197-0
  5. Harrison, A. P. 1995. Survival of bacteria upon repeated freezing and thawing. J. Bacteriol. 70: 711-715
  6. Henry, J. and Kirsop, B. 1989. Cryopreservation of yeasts in polypropylene straws. World Federation for Culture Collection Technical Information Sheet No.3
  7. Hoffmann, P. 1989. Cryopreservation of fungi. World Federation for Culture Collection - Technical Information Sheet No.5
  8. Hwang, S.- W. 1966. Long-term preservation of fungus cultures with liquid nitrogen refrigeration. Appl. Microbiol. 14: 784-788
  9. Hwang, S.-W. 1968. Investigation of ultra-low temperature for fungal cultures. I. Evaluation of liquid-nitrogen storage for preservation of selected fungal cultures. Mycologia 60: 613-621 https://doi.org/10.2307/3757428
  10. Hwang, S.-W, Kwolek, W. F. and Haynes, W. C. 1976. Investigation of ultra-low temperature for fungal cultures. III. Viability and growth rate of mycelial cultures following cryogenic storage. Mycologia 68: 377-387 https://doi.org/10.2307/3759008
  11. Nakasone, K. K., Peterson, S. W. and Jong, S. C. 2004. Preservation and distribution of fungal cultures. Pp 37-47. In: Mueller G M., Bills, G F. and Foster, M. S. Eds. Biodiversity of fungi - Inventory and monitoring methods. Elsevier Academic Press, San Diego
  12. Ohmasa, M., Abe, Y., Babasaki, K., Hiraide, M. and Okabe, K. 1992. Preservation of cultures of mushrooms by freezing. Trans. Mycol. Soc. Jap. 33: 467-479
  13. Simione, F. P., Daggett, P. M., McGrath, M. S. and Alexander, M. T. 1977. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502 https://doi.org/10.1016/0011-2240(77)90013-X
  14. Smith, D. 1983. Cryoprotectants and the cryopreservation of fungi. Trans. Brit. Mycol. Soc. 80: 360-363 https://doi.org/10.1016/S0007-1536(83)80025-4
  15. Smith, D. 1991. Maintenance of filamentous fungi. Pp 133-159. In: Kirsop, B. E. and Doyle, A. Eds. Maintenance of microorganisms and cultured cells - A manual of laboratory methods. Academic Press, San Diego
  16. Staplers, J. A., de Hoog, A. and Vlug, I. J. 1987. Improvement of the straw technique for the preservation of fungi in liquid nitrogen. Mycologia 79: 82-89 https://doi.org/10.2307/3807747
  17. Tuite, J. 1968. Liquid nitrogen storage of fungi sealed in a polyester film. Mycologia 60: 591-594 https://doi.org/10.2307/3757425

Cited by

  1. Maintenance of Filamentous Fungi in Korean Agricultural Culture Collection (KACC) vol.42, pp.2, 2014, https://doi.org/10.4489/KJM.2014.42.2.97