Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

Effects of Hwadamtongrak-Tang on the changes of Cerebral Flow in the rats

화담통락탕(化痰通絡湯)이 백서의 뇌혈류변화 및 허혈성 뇌손상에 미치는 영향

  • Park, Hyoung-Bae (Department of Internal Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Yang, Seung-Jung (Sun Cheon Oriental Medicine Hospital) ;
  • Wei, Tung-Sheun (Sun Cheon Oriental Medicine Hospital) ;
  • Park, Hye-Sun (Sun Cheon Oriental Medicine Hospital) ;
  • Jeon, Sang-Yoon (Department of Internal Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Hong, Seok (Department of Internal Medicine, College of Oriental Medicine, Dong-Shin University)
  • 박형배 (동신대학교 한의과대학 내과학교실) ;
  • 양승정 (동신대학교 순천한방병원) ;
  • 위통순 (동신대학교 순천한방병원) ;
  • 박혜선 (동신대학교 순천한방병원) ;
  • 전상윤 (동신대학교 한의과대학 내과학교실) ;
  • 홍석 (동신대학교 한의과대학 내과학교실)
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives & Methods : This present study was performed to investigate the effect of Hwadamtongrak-Tang extract (HTT) on the regulation of cerebral hemodynamics in terms of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP)] in normal and cerebral ischemic rats. Also the effects of HTT on changes in local blood flow, inhibition of LD H activity in neuronal cells, and levels of cytokine production in the serum were determined in the ischemic rat model. The major findings are summarized below. Results : 1. HTT significantly increased rCBF in a dose-dependent manner, but MABP was not changed by HTT treatment. These results suggest that HTT may increase rCBF by dilating cerebral arterial diameter. 2. HTT-induced increase in rCBF was blocked by pretreatment with cyclooxygenase inhibitor indomethacin (IDN, 1 mg/kg, i.p.) and MABP was significantly increased by ID N. 3. Pretreatment of methylene blue $(MTB,\;10\;{\mu}g/kg,\;i.p.)$, an inhibitor of guanylate cyclase, significantly decreased both rCBP and MABP in HTT-treated rats. 4. HTT treatment significantly increased rCBP to a stable level during the period of cerebral reperfusion. 5. HTT significantly inhibited LD H activity in neuronal cells, suggesting a neuroprotection by HTT. 6. Serum interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ levels were significantly decreased in the femoral artery 1 hr after middle cerebral arterial occlusion in HTT-treated rats. IL-10 levels in the serum were significantly increased by HTT treatment whereas transforming growth factor $(TGF)-{\beta}$ levels were similar between HTT-treated and control groups. 7. Serum interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ levels were significantly decreased in the femoral artery 1 hr after reperfusion in HTT-treated rats. Serum IL-10 levels were significantly decreased in HTT-treated rats compared with the control group, and no significant changes in $(TGF)-{\beta}$ in the serum were observed by HTT treatment. Conclusions: The present data suggest that HTT may have an anti-ischemic effect via the improvement of cerebral hemodynamics and thus protect the brain from ischemic damage.

Keywords