Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

The effects of Chenwangbosim-Dan and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation

천왕보심단(天王補心丹)과 단미(單味)들이 Hypoxia-Reoxygenation에 의해 손상받은 Mouse Neuroblastoma 2a Cells에미치는 영향(影響)

  • Kim, Sang-Ho (Dept. of Neuropsychiatry, Collage of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jong-Woo (Dept. of Neuropsychiatry, Collage of Oriental Medicine, Kyung-Hee University) ;
  • Kang, Chul-Hun (Graduate School of East-West Medical Science) ;
  • Whang, Wei-Wan (Dept. of Neuropsychiatry, Collage of Oriental Medicine, Kyung-Hee University)
  • 김상호 (경희대학교 한의과대학 신경정신의학교실) ;
  • 김종우 (경희대학교 한의과대학 신경정신의학교실) ;
  • 강철훈 (경희대학교 동서의학대학원) ;
  • 황의완 (경희대학교 한의과대학 신경정신의학교실)
  • Published : 2006.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was designed to assess effect of and Chenwangbosim-Dan(CWBSD) herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by CWBSD and herbs. Result : 1. In MTT assay of hypoxia CWBSD and BJI, SJH, IS, CHR, HS among all of herbs were effective. Especially CWBSD and IS were highly effective. 2. In MTT assay of reoxygenation SJI, SJH, VJ, IS, BJI were effective. Especially SJI, SJH, YJ were highly effective. 3. In LDH assay of hypoxia CWBSD, DS, DG, SJH, OMZ were effective. Especially CWBSD, DG were highly effective. 4. In LDH assay of reoxygenation all of herbs except CWBSD and BJI were generally effective. Especially CHR, SJH, YJ, OMZ, HS were highly effective. Conclusion : The results suggest that CWBSD, and it's ingradient(especially SJH, CHR and SJI) may have protective effect on condition of oxidative stress.

Keywords