Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Differential Expression of Gangliosides in the Ovary and Uterus of Streptozotocin-Induced and db/db Diabetic Mice

  • Kim, Sung-Min (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Kwak, Dong-Hoon (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Kim, Sun-Mi (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Jung, Ji-Ung (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Lee, Dae-Hoon (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Lee, Seoul (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Jung, Kyu-Yong (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Do, Su-Il (Department of Life Science, Ajou University) ;
  • Choo, Young-Kug (Department of Biological Science, College of Natural Sciences, Wonkwang University)
  • Published : 2006.08.01
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Abstract

Gangliosides are widely distributed in mammalian cells and play important roles in various functions such as cell differentiation and growth control. In addition, diabetes and obesity cause abnormal development of reproductive processes in a variety of species. However, the mechanisms underlying these effects, and how they are related, are not fully understood. This study examined whether the differential expression of gangliosides is implicated in the abnormal follicular development and uterine architecture of streptozotocin (STZ)-induced and db/db diabetic mice. Based upon the mobility on high-performance thin-layer chromatography, mouse ovary consisted of at least five different ganglioside components, mainly gangliosides GM3, GM1, GD1a and GT1b, and diabetic ovary exhibited a significant reduction in ganglioside expression with apparent changes in the major gangliosides. A prominent immunofluorescence microscopy showed a dramatic loss of ganglioside GD1a expression in the primary, secondary and Graafian follicles of STZ-induced and db/db diabetic mice. A significant decrease in ganglioside GD3 expression was also observed in the ovary of db/db mice. In the uterus of STZ-induced diabetic mice, expression of gangliosides GD1a and GT1b was obviously reduced, but gangliosides GM1, GM2 and GD3 expression was increased. In contrast, the uterus of db/db mice showed a significant increase in gangliosides GM1, GD1a and GD3 expression. Taken together, a complex pattern of ganglioside expression was seen in the ovary and uterus of normoglycemic ICR and $db/^+$ mice, and the correspoding tissues in diabetic mice are characterized by appreciable changes of the major ganglioside expression. These results suggest that alterations in ganglioside expression caused by diabetes mellitus may be implicated in abnormal ovarian development and uterine structure.

Keywords

References

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