Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Effect of Additives on the Viability of Bifidobacteria Loaded in Alginate Poly-l-lysine Microparticles during the Freeze-drying Process

  • Cui, Jing-Hao (National Research Laboratory for Bioavailability Control, College of Pharmacy, Kangwon National University,College of Pharmacy, Soochow University) ;
  • Cao, Qing-Ri (National Research Laboratory for Bioavailability Control, College of Pharmacy, Kangwon National University) ;
  • Choi, Yun-Jaie (College of Agricultural Life Science, Seoul National University) ;
  • Lee, Kyung-Hoon (Department of Molecular Cell Biology, School of Medicine, Sungkyunkwan University, Clinical Research Institute, Samsung Medical Center) ;
  • Lee, Beom-Jin (National Research Laboratory for Bioavailability Control, College of Pharmacy, Kangwon National University)
  • Published : 2006.08.01
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Abstract

Bifidobacteria-loaded alginate poly-l-lysine microparticles (bap microparticles) were prepared using an air atomization method and then freeze-dried. The viability of the bap microparticles was investigated as a function of the amount of the bifidobacteria cultures, and the addition of a yeast extract, cryoprotectants, antioxidants and neutralizer. The size of the bap microparticles with and without the bifidobacteria was $84.8{\pm}28.5\;{\mu}m$ ($mean{\pm}standard$ deviation) and $113.1{\pm}38.5\;{\mu}m$, respectively. The surface morphology was slightly ellipsoid and wrinkled regardless of the incorporating bifidobacteria. The viability gradually decreased with increasing freeze-drying time. Free-flowing powdered bap microparticles were obtained at least 12 h after freeze-drying the wetted slurry of bap microparticles. However, the particles tended to aggregate when either lactose or ascorbic acid was added. The addition of a yeast extract, cryoprotectants (glycerol and lactose), antioxidants ($NaHSO_3$ and ascorbic acid) and neutralizer $(Mg_3(PO_4)_2)$ resulted in a significantly higher viability of the bifidobacteria in the bap microparticles after freeze-drying (0.34-1.84 log) compared with the culture alone.

Keywords

References

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