YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Apoptotic Effect of Ethanol Extracts of Bojungbangamtang and Acidic Polysaccharide of Korea Red Ginseng in a MCF7/adR Multidrug-resistance Breast Cancer Cells

항암제 내성 유방암 MCF7/adR 세포주에 대한 보정방암탕과 홍삼산성다당체의 세포고사 유도효과

  • Ahn, Gyu-In (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Park, Cheol-Hwan (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee, Eun-Ok (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee, Hyo-Jung (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee, Jae-Ho (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim, Kwan-Hyun (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Rhee, Yun-Hee (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Jang, Yu-Sung (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim, Sang-Tae (Nano-Bio of Research Institute, Division of Nanoscience, Ewah Womans University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyunghee University,College of Oriental Medicine, Kyunghee University)
  • 안귀인 (경희대학교 동서의학대학원) ;
  • 박철환 (경희대학교 동서의학대학원) ;
  • 이은옥 (경희대학교 동서의학대학원) ;
  • 이효정 (경희대학교 동서의학대학원) ;
  • 이재호 (경희대학교 동서의학대학원) ;
  • 김관현 (경희대학교 동서의학대학원) ;
  • 이연희 (경희대학교 동서의학대학원) ;
  • 장유성 (경희대학교 동서의학대학원) ;
  • 김상태 (이화여자대학교 나노과학부 나노바이오연구소) ;
  • 김성훈 (경희대학교 동서의학대학원,경희대학교 한의과대학)
  • Published : 2006.08.31
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Abstract

This study was undertaken to determine whether the 9 herbal complex induces apoptosis in human breast cancer MCF-7 cells and adriamycin-resistant MCF7/adR cells. Ethanol extracts of Bojungbangamtang (BBTE) and acidic polysaccharide of Red Ginseng (GIN) induced cell death in both MCF-7 and MCF7/adR cells. Ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng also induced $G_2/M$ cell cycle arrest and increased TUNEL positive cells in MCF7/adR cells. In addition, flow cytometric analysis revealed the decreased expression of P-glycoprotein (P-gp) in ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng treated MCF7/adR cells. Similarly, decreased protein levels of P-glycoprotein and multidrug resistance associated proteins-1 were also determined by immunocytometry in ethanol extracts of Bojungbangamtang treated MCF7/adR cells. Taken together these data indicate that ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng inhibit the function of ABC transporters such as multi drug resistance associated proteins (MRPs) and P-glycoprotein as well as induce apoptosis in MCF7/adR cells. Thus, these data suggest that ethanol extracts of Bojungbangamtang and polysaccharide of Red Ginseng can be candidates for the treatment of multidrug-resistant MCF7/adR cells.

Keywords

References

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