YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Identification of Proteins Interacting with CD1d

CD1d와 상호작용하는 단백질의 동정

  • Published : 2006.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

CD1d is an unique antigen presenting molecule which provides antigenic repertoires to NKT cells. To examine molecules required for CD1d antigen presentation, we determined an interaction between CD1d and several endoplasmic reticulum (ER) resident molecular chaperones by co-immunoprecipitation. Results indicated that calnexin and calreticulin seem to be bound to mouse CD1d, but TAP and tapasin do not bind. Further, we screened an yeat two hybrid system to identify proteins that help mouse CD1d transportation in the cytosol. We found that two proteins, heat shock protein a sub-unit $(Hsp90{\alpha})$ and protein kinase C and casein kinase substrate in neurons 3 (PACSIN-3), interact with CD1d. Future study will be focus on the role of these molecules during the CD1d antigen presentation.

Keywords

References

  1. Beckman, E. M., Porcelli, S. A., Morita, C. T., Behar, S. M., Furlong, S. T. and Brenner, M. B. : Recognition of a lipid antigen by CD1-restricted $\alpha\beta$+T cells. Nature 372, 691 (1994) https://doi.org/10.1038/372691a0
  2. Bendelac, A., Rivera, M. N., Park, S. H. and Roark, J. H. : Mouse CD1-specific NK1.1+ T cells: development, specificity, and function. Annu. Rev. Immunol. 15, 535 (1997) https://doi.org/10.1146/annurev.immunol.15.1.535
  3. Taniguchi, M. and Nakayama, T. : Recognition and function of V$\alpha$14 NKT cells. Semin. Immunol. 12, 543 (2000) https://doi.org/10.1006/smim.2000.0270
  4. Kawano, T., Cui, J., Koezuka, Y., Toura, I., Kaneko, Y., Motoki, K., Ueno, H., Nakagawa, R., Sato, H., Kondo, E., Koseki, H. and Taniguchi, M. : CD1d-restricted and TCR-mediated activation of VSemin. Immunol.14 NKT cells by glycosylceramides. Science 278, 1626 (1997) https://doi.org/10.1126/science.278.5343.1626
  5. Crompton, T., Pircher, H. and MacDonald, H. R. : CD4+8- thymocytes bearing major histocompatibility complex class Irestricted T cell receptors: evidence for homeostatic control of early stages of CD4/CD8 lineage development. J. Exp. Med. 176, 903 (1992) https://doi.org/10.1084/jem.176.3.903
  6. Eberl, G., Lees, R., Smiley, S. T., Taniguchi, M., Grusby, M. J. and MacDonald, H. R. : Tissue-specific segregation of CD1ddependent and CD1d-independent NK T cells. J. Immunol. 162, 6410 (1999)
  7. Bannai, M., Kawamura, T., Naito, T., Kameyama, H., Abe, T., Kawamura, H., Tsukada, C., Watanabe, H., Hatakeyama, K., Hamada, H., Nishiyama, Y., Ishikawa, H., Takeda, K., Okumura, K., Taniguchi, M. and Abo, T. : Abundance of unconventional CD8(+) natural killer T cells in the large intestine. Eur. J. Immunol. 31, 3361 (2001) https://doi.org/10.1002/1521-4141(200111)31:11<3361::AID-IMMU3361>3.0.CO;2-Z
  8. Cui, J., Shin, T., Kawano, T., Sato, H., Kondo, E., Toura, I., Kaneko, Y., Koseki, H., Kanno, M. and Taniguchi, M. : Requirement for V$\alpha$14 NKT cells in IL-12-mediated rejection of tumors. Science 278, 1623 (1997) https://doi.org/10.1126/science.278.5343.1623
  9. Hammond, K. J. and Godfrey, D. I. : NKT cells: Potential targets for autoimmune disease therapy- Tissue Antigens 59, 353 (2002) https://doi.org/10.1034/j.1399-0039.2002.590501.x
  10. Prigozy, T. I., Sieling, P. A., Clemens, D., Stewart, P. L., Behar, S. M., Porcelli, S. A., Brenner M. B., Modlin, R. L. and Kronenberg, M. : The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. Immunity 6, 187 (1997) https://doi.org/10.1016/S1074-7613(00)80425-2
  11. Chiu, Y. H., Park, S. H., Benlagha, K., Forestier, C., Jayawardena-Wolf, J., Savage, P. B., Teyton, L. and Bendelac, A. : Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CD1d. Nat. Immunol. 3, 55 (2002) https://doi.org/10.1038/ni740
  12. Pamer, E. and Cresswell, P. : Mechanisms of MHC classIrelated antigen processing. Annu. Rev. Immunol. 16, 323 (1998) https://doi.org/10.1146/annurev.immunol.16.1.323
  13. Smith-Jones, P. M., Solit, D. B., Akhurst, T., Afroze, F., Rosen, N. and Larson, S. M. : Imaging the pharmacodynamics of HER2 degradation in response to Hsp90 inhibitors. Nat. Biotechnol. 22, 701 (2004) https://doi.org/10.1038/nbt968
  14. Modregger, J., Ritter, B., Witter, B., Paulsson, M. and Plomann M. : All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis. J. Cell Sci. 113, 4511 (2000)