The Korea Journal of Herbology (대한본초학회지)
- Volume 21 Issue 2
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- Pages.165-173
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- 2006
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
Inhibitory effects of Coptidis Rhizoma on the LPS-induced production of nitric oxide and $TNF-{\alpha}$ in mouse macrophage cells
황련의 쥐 대식세포로부터 LPS에 의해 유도되는 nitric oxide 및 $TNF-{\alpha}$ 의 생성억제효과
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Jung, Hyo-Won
(Dept. of Herbology, College of Oriental Medicine, Dongguk University) ;
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Park, Yong-Ki
(Dept. of Herbology, College of Oriental Medicine, Dongguk University)
- Published : 2006.06.30
Abstract
Objectives : Coptidis Rhizoma has been known traditional medicine with antimicrobial activities. We investigated inhibitory effects of Coptidis Rhizoma extract on lipopolysaccharide(LPS)-induced nitric oxide production from mouse macrophages. Methods : After Coptidis Rhizoma extract was pretreated in BV2, mouse brain macrophages and RAW264.7 mouse macrophages, cells were activated with LPS. To investigate cytotoxicity Coptidis Rhizoma extract, cell viability was measured by MTT assay. The production of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) was determined in each culture supernatant and mRNA by Griess reaction and RT-PCR. The production of
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