The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Tyronase Inhibitory Effect of 3,4-Dihydroxybenzaldehyde Isolated from Pinellia ternata

반하에서 3,4-Dihydroxybenzaldehyde의 분리, 구조동정 및 Tyrosinase 활성 저해효과

  • Shin, Dong-Soo (Department of Chemistry College of Natural Sciences, Changwon National University) ;
  • Paik, Do-Hyeon (Department of Biochemistry and Health science, Changwon National University) ;
  • Yoon, Do-Young (Department of Biosci. & Biotechnology, Konkuk University) ;
  • Shin, Dong-Min (Kukje Oriental Medicine) ;
  • Cho, Yong-Kweon (Department of Biochemistry and Health science, Changwon National University)
  • 신동수 (창원대학교 자연과학대학 화학과) ;
  • 백도현 (창원대학교 자연과학대학 보건의과학과) ;
  • 윤도영 (건국대학교 분자생명공학과) ;
  • 신동민 (경남 마산시 석전동 국제한의원) ;
  • 조용권 (창원대학교 자연과학대학 보건의과학과)
  • Published : 2006.06.30
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Abstract

Objectives : The purpose of this study is to isolate tyrosinase inhibitory material from Pinellia ternata and characterize its own structure and activity. Methods : Pinellia ternata (600g) was extracted with 95% methanol (1L) at $37^{\circ}C$ for 4 days, with shaking at 250rpm. The extract was further solvent-fractionated with n-hexane, chloroform, ethylacetate and water. The active fraction was subjected to JAI recycling prep-HPLC JAIGEL GS-320 column. The structure was identified for the active peak with NMR and GC. Results : Tyrosinase was potently inhibited by 95% methanol extracts from Pinellia ternata. The $IC_{50}$ value of the extracts was estimated to be 0.05mg/ml. The extracts was divided into four solvent-fractions, and the most potent tyrosinase inhibition was found in ethylacetate layer. $IC_{50}$ value of ethylacetate fraction was 0.001mg/ml. This fraction was further purified with JAI Recycling Preparative HPLC (Model: LC 9104). The isolated compound showing inhibitory activity was characterized on its chemical structure by NMR and the compound was identified as 3,4-dihydroxybenzaldehyde. $IC_{50}$ was found to be 7.74 ${\mu}M$ which is much lower than that of kojic acid $(66.5{\mu}M)$. Conclusions : The data suggest that 3,4-dihydroxybenzaldehyde isolated and identified from Pinellia ternata is very strong inhibitor to melanin biosynthesis.

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