Apoptotic pathway of SNU-1, human gastric cancer cell line, by Bodusan

보두산에 의한 사람 위암 세포주 SNU-1의 세포사멸 경로

  • Lee, Jae-Eun (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Joung (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University) ;
  • Lee, Young-Tae (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University) ;
  • Bae, Chang-Wook (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University) ;
  • Jeun, Hyun-Sook (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Department of Herbal pharmacology, Collage of Oriental Medicine, Dongguk University)
  • 이재은 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 방제학교실) ;
  • 이영태 (동국대학교 한의과대학 방제학교실) ;
  • 배창욱 (동국대학교 한의과대학 방제학교실) ;
  • 전현숙 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Published : 2006.03.30

Abstract

Objectives : The purpose of this study was to investigate the anticancer effects of Bodusan (BDS) on SNU-1 cells, a human gastric cancer cell line. Methods : To study the cytotoxic effect of BDS on SNU-1 cells, the cells were treated with various concentrations of BDS and then cell viability was determined by XTT reduction method and trypan blue exclusion assay. The typical signs of apoptosis, was examined by western blot analysis. BDS-induced MAPK activation was also examined by Western blot for phosphorylated ERK and p38. Results : BDS reduced proliferation of SNU-1 cells in a dose-dependent manner and decreased procaspase 3 level in a dose-dependent manner and induced the clevage of PARP at concentration > 500 ${\mu}g/ml$. BDS also triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome C from mitochondria to cytosol and reducing the level of anti-apoptotic Bcl-2. BDS significantly decreased ERK phosphorylation and increased p38 phosphorylation in a dose-dependent manner. Futhermore, BDS treatment up-regulated p53 and p21waf expression in a dose-dependent manner. Conclusion : BDS-induced apoptosis is MAP kinase-dependent apoptoric pathway and arrested SNU-1 cells at the G0/G1 of cell cycle. These results suggest that BDS is potentially useful as a chemotherapeutic agent in human gastric cancer.

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