Korean Journal of Acupuncture
- 제23권3호
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- Pages.81-98
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- 2006
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- 2287-3368(pISSN)
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- 2287-3376(eISSN)
태위(太衝)(LR3)에 대한 영수(迎隨)및 염전보사(捻轉補瀉)가 intraluminal filament 삽입술(揷入術)에 의하여 유발(誘發)된 백서(白鼠)의 focal ischemia에 미치는 영향(影響)
Anti-apoptotic and neuroprotective effects of acupuncture techniques of tonification or sedation at LR3 on focal brain ischemic injury induced by intraluminal filament insertion in rats
- 박종승 (동신대학교 한의과대학 경혈학교실) ;
-
나창수
(동신대학교 한의과대학 경혈학교실) ;
-
조명래
(동신대학교 한의과대학 침구학교실) ;
- 정연진 (전남대학교 치의학전문대학원 치의학연구소) ;
- 정지연 (전남대학교 치의학전문대학원 치의학연구소) ;
- 김원재 (전남대학교 치의학전문대학원 치의학연구소) ;
-
최찬헌
(동신대학교 한의과대학 생리학교실) ;
-
윤대환
(동신대학교 한의과대학 경혈학교실)
- Park, Jong-Seung (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University) ;
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Na, Chang-Su
(Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University) ;
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Cho, Myeng-Rae
(Dept. of Meridian and Acupoint, College of Oriental Medicine, Dong-Shin University) ;
- Jung, Yeon-Jin (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
- Jeong, Ji-Yeon (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
- Kim, Won-Jae (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
-
Choi, Chan-Hun
(Dept. of Oriental Medicine College of Oriental Medicine, Dong-Shin University) ;
-
Youn, Dae-Hwan
(Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University)
- 발행 : 2006.09.27
초록
Objectives : Acupuncture using a tonification or sedation techniques method is used as a controlling the medication for an early stroke in the Korean medicine. LR3 has indicatons of headache, vertigo, facial paralysis, apoplexy, epiepsy as the source acupoint of a liver meridian. So this study is aims to investigate the anti-apoptotic and neuroprotective effects of acupuncture on the focal ischemia induced by intraluminal filament insertion in rats. Methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The animals were divided into seven groups (n=8 in each group) : Normal, intactness group; Conrol group, no therapy group after being ischemia induced; MA-l, acupuncture perpendicularly without Tonification or Sedation techniques at LR3 after being ischemia induced; MA-2, acupuncture obliquely towards the knee at LR3 after being ischemia induced; MA-3, acupuncture obliquely towards the toe at LR3 after being ischemia induced; MA-4, acupuncture obliquely towards the knee and rotate 9 times in a clockwise direction at LR3 after being ischemia induced; MA-5, acupuncture obliquely towards the toe and rotate 6 times in a counterclockwise direction at LR3 after being ischemia induced. The anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR3 are observed by mGluR5, Bax, Cresyl violet, ChAT-stain and NGF. Results : The intensity of mGluR5 and the density of ChAT was increased in MA-1 group. The intensity of Bax was decreased in MA-3, MA-4 group. The density of neurons stained by Cresyl violet and ChAT was increased in MA-2, MA-3, MA-4, MA-5 group. The density of neurons stained by NGF was only increased in MA-4 group. Conclusions : Our study suggests that acupuncture perpendicularly without Tonification or Sedation techniques and obliquely towards the knee and rotate 9 times in a clockwise direction(Tonifying technique) at LR3 after being ischemia induced at LR3 shows anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.