Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

[Ru(phen)2(dppz)]2+ Assemble on the Surface of the SDS Micelle and Its Application for the Determination of DNA

  • Chen, Fang (Department of Chemistry, Huazhong University of Science & Technology) ;
  • Huang, Jianping (College of Chemistry & Environmental Engineering, Yangtze University) ;
  • He, Zhike (College of Chemistry & Molecular Sciences, Wuhan University)
  • Published : 2006.10.20
Download PDF
⟨ Previous Next ⟩

Abstract

The solution of $[Ru(phen)_2(dppz)]^{2+}$ and SDS has high Resonance Light Scattering (RLS) signals due to $[Ru(phen)_2(dppz)]^{2+}$ assemble on the surface of the SDS micelle. Because of the high affinity ($KB\geq10^6\;L\;mol^{-1}$) between $[Ru(phen)_2(dppz)]^{2+}$ and DNA, the adding of DNA in the solution of $[Ru(phen)_2(dppz)]^{2+}$-SDS makes the dissociation of $[Ru(phen)_2(dppz)]^{2+}$-SDS, and results in decreasing of the RLS signals and increasing of the absorbance. Based on this, a novel method is proposed for DNA assay. Under optimum condition, good linear relationship was obtained within the concentration range of 0.018-1.26 $\mu g\;mL^{-1}$, the linear equation is $I_{RLS}$ = 504.8-348.8 c (c: $\mu g\;mL^{-1}$) and the correlation coefficient (r) is 0.9992. The detect limit for calf thymus DNA is 8.6 ng $mL^{-1}$. The proposed method was successful applied to determine the extracted colibacillus plasmid DNA.

Keywords

References

  1. Friedman, A. E.; Chambron, J. C.; Sauvage, J. P.; Turro, N. J.; Barton, J. K. J. Am. Chem. Soc. 1990, 112, 4960 https://doi.org/10.1021/ja00168a052
  2. Maheswari, P. U.; Rajendiran, V.; Palaniandavar, M.; Parthasarathi, R.; Subramanian, V. J. Inorg. Biochem. 2006, 100, 3 https://doi.org/10.1016/j.jinorgbio.2005.09.008
  3. Moucheron, C.; Mesmaeker, A. K.; Choua, S. Inorg. Chem. 1997, 36, 584 https://doi.org/10.1021/ic9609315
  4. Zou, X. H.; Ye, B. H.; Li, H.; Zhang, Q. L.; Chao, H.; Liu, J. G.; Ji, L. N.; Li, X. Y. J. Biol. Inorg. Chem. 2001, 6, 143 https://doi.org/10.1007/s007750000183
  5. Holmlin, R. E.; Barton, J. K. Inorg. Chem. 1995, 34, 7 https://doi.org/10.1021/ic00105a004
  6. Westerlund, F.; Wilhelmsson, L. M.; Norden, B.; Lincoln J. Phys. Chem. B 2005, 109, 21140 https://doi.org/10.1021/jp0534838
  7. Haq, I.; Lincoln, P.; Suh, D.; Norden, B.; Chowdhry, B. Z.; Chaires, J. B. J. Am. Chem. Soc. 1995, 117, 4788 https://doi.org/10.1021/ja00122a008
  8. Nordell, P.; Lincoln, P. J. Am. Chem. Soc. 2005, 127, 9670 https://doi.org/10.1021/ja0521674
  9. Olson, E. J. C.; Hu, D.; Hormann, A.; Jonkman, A. M.; Arkin, M. R.; Stemp, E. D. A.; Barton, J. K.; Barbara, P. F. J. Am. Chem. Soc. 1997, 119, 11458 https://doi.org/10.1021/ja963244l
  10. Coates, C. G.; McGarvey, J. J.; Callaghan, P. L.; Coletti, M.; Hamilton, J. G. J. Phys. Chem. B 2001, 105, 730 https://doi.org/10.1021/jp002856w
  11. Ling, L. S.; He, Z. K.; Song, G. W.; Zeng, Y. E. et al. Anal. Chim. Acta 2001, 436, 207 https://doi.org/10.1016/S0003-2670(01)00922-9
  12. Chen, F.; Ai, X. P.; He, Z. K.; Li, M. J.; Chen, X. D. Spectro. Lett. 2005, 38, 99 https://doi.org/10.1081/SL-200049565
  13. Ling, L. S.; He, Z. K.; Chen, F.; Zeng, Y. E. Talanta 2003, 59, 269 https://doi.org/10.1016/S0039-9140(02)00499-X
  14. Ling, L. S.; He, Z. K.; Chen, F.; Zhang, H. S.; Zeng, Y. E. Chin. Chem. Lett. 2003, 14, 30
  15. Jiang, Y. X.; Fang, X. H.; Bai, C. L. Anal. Chem. 2004, 76, 5230 https://doi.org/10.1021/ac049565u
  16. Carter, J. C.; Egan, W. J.; Nair, R. B.; Murphy, C. J.; Morgan, S. L.; Angel, S. M. Book of Abstracts, PITTCON'99 (The Pittsburgh Conference) 1999, 1143
  17. Huang, C. Z.; Li, K. A.; Tong, S. Y. Anal. Chem. 1996, 68, 2259 https://doi.org/10.1021/ac9511105
  18. Chen, Z. G.; Ding, W. F.; Ren, F. L.; Han, Y. L.; Liu, J. B. Anal. Lett. 2005, 38, 2301 https://doi.org/10.1080/00032710500316217
  19. Chen, F.; Huang, J. P.; Ai, X. P.; He, Z. K. Analyst 2003, 128, 1462 https://doi.org/10.1039/b309625d
  20. Ling, L. S.; Song, G. W.; He, Z. K.; Liu, H. Z.; Zeng, Y. E. Microchem. J. 1999, 63, 356 https://doi.org/10.1006/mchj.1999.1741
  21. Chaires, J. B.; Dattagupta, N.; Crothers, D. M. Biochem. 1982, 21, 3933 https://doi.org/10.1021/bi00260a005
  22. Wilson, W. D.; Tanious, F. A.; Watson, R. A.; Barton, H. J.; Atrekowska, A.; Harden, D. B.; Strekowski, L. Biochem. 1989, 28, 1984 https://doi.org/10.1021/bi00431a005
  23. Westerlund, F.; Wilhelsson, L. M.; Norden, B.; Lincoln, P. J. Am. Chem. Soc. 2003, 125, 3773 https://doi.org/10.1021/ja029243c

Cited by

  1. A theoretical study on the substituent effect of DNA-photocleavage by [Ru(phen)2(6-R-dppz)]2+ (R=H, OH, and NO2) vol.961, pp.1, 2006, https://doi.org/10.1016/j.theochem.2010.08.030