Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Purification and Characterization of Substance P-related Peptide from the Body of the African Lungfish, Protopterus dolloi

  • Kim, Chan-Hee (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Eun-Jung (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Go, Hye-Jin (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Lee, Hyung-Ho (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Hong, Yong-Ki (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Hyung-Rak (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Chung, Joon-Ki (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Jang-Su (Department of Chemistry, College of Natural Science, Pusan National University) ;
  • Muneoka, Yojiro (Faculty of Integrated Arts and Sciences, Hiroshima University) ;
  • Park, Nam-Gyu (Faculty of Food Science and Biotechnology, Pukyong National University)
  • Published : 2006.01.20
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Abstract

The peptide with structural similarity to mammalian substance P (M-SP) has been isolated from extract of the body of the African lungfish, Protopterus dolloi, using the rectum of the newt as the bioassay system. The primary structure of the SP-related peptide was identified as Lys-Pro-Arg-Pro-Asp-Gln-Phe-Tyr-Gly-Leu-Met-NH2 (L-SP) and contained four substitutions ($Lys^{1}\rightarrow $ Arg, $Arg^{3}\rightarrow$ Lys, $Asp^{5}\rightarrow$ Gln, and $Tyr^{8}\rightarrow$ Phe) compared with M-SP; this structure is identical to that of the peptide isolated from the gut of the Australian lungfish. Circular dichroism spectra showed that L-SP had an unordered structure in the buffer solution and phospholipid bilayers. This peptide was found to have an excitatory effect on rectal muscle tissues of newt, quail, and fish. L-SP also had a more potent vasodilatory effect on the guinea-pig aorta than that of M-SP. The identification of the peptide provides evidence that SP family, hitherto confined to mammals, have a widespread occurrence in lungfish.

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References

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