Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Purification and Characterization of [Ala2]-Neuromedin N from the Visceral Tissue of the African Lungfish, Protopterus dolloi

  • Kim, Chan-Hee (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Go, Hye-Jin (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Kim, Eun-Jung (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Seo, Jung-Kil (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Hong, Yong-Ki (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Kim, Hyung-Rak (Faculty of Food Science & Biotechnology, Pukyong National University) ;
  • Chung, Joon-Ki (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Muneoka, Yojiro (Faculty of Integrated Arts and Sciences, Hiroshima University) ;
  • Park, Nam-Gyu (Faculty of Food Science & Biotechnology, Pukyong National University)
  • Published : 2006.11.20
Download PDF
⟨ Previous Next ⟩

Abstract

A new biologically active peptide with structural similarity to neuromedin N (NMN) has been isolated from extracts of visceral tissue of the African lungfish, Protopterus dolloi, using the rectum of the quail as the bioassay system. The primary structure of NMN-related peptide was established as Lys-Ala-Pro-Tyr-Ile-Leu-OH ([$Ala_2$]-NMN) and contained one substitution ($Ala_2\rightarrow$Ile) compared with the porcine NMN. [$Ala_2$]-NMN was found to have an excitatory effect on rectal muscle tissues of quail (Coturnix japonica), newt (Cynops pyrrhogaster) and black bass (Micropterus sulmoides). The threshold concentration of [Ala2]-NMN for contraction of C. japonica muscle was found to be approximately $10^-11$M. [$Ala_2$]-NMN showed contractile activities in the following order: C. japonica > C. pyrrhogaster > M. sulmoides. The identification of [Ala2]-NMN provides evidence that NMN family, hitherto confined to mammals, has a widespread occurrence in lungfish.

Keywords

References

  1. Minamino, N.; Kangawa, K.; Matsuo, H. Biochem. Biophys. Res. Commun. 1984, 122, 542-549 https://doi.org/10.1016/S0006-291X(84)80067-4
  2. Carraway, R.; Leeman, S. E. J. Biol. Chem. 1973, 248, 6854-6861
  3. Dobner, P. R.; Barber, D. L.; Villa-Komaroff, L.; McKiernan, C. Proc. Natl. Acad. Sci. USA 1987, 84, 3516-3520 https://doi.org/10.1073/pnas.84.10.3516
  4. Kislauskis, E.; Bullock, B.; McNeil, S.; Dobner, P. R. J. Biol. Chem. 1988, 263, 4963-4968
  5. Carraway, R. E.; Ruane, S. E.; Ritsema, R. S. Peptides 1983, 4, 111-116 https://doi.org/10.1016/0196-9781(83)90176-6
  6. Carraway, R. E.; Mitra, S. P. Endocrinology 1987, 120, 2092- 2100 https://doi.org/10.1210/endo-120-5-2092
  7. Eldred, W. D.; Li, H. B.; Carraway, R. E.; Dowling, J. E. Brain Res. 1987, 424, 361-370 https://doi.org/10.1016/0006-8993(87)91481-8
  8. Lee, Y. C.; Ball, J. A.; Reece, D.; Bloom, S. R. FEBS Lett. 1987, 220, 243-246 https://doi.org/10.1016/0014-5793(87)80913-4
  9. Reiner, A. Brain Res. 1987, 422, 186-191 https://doi.org/10.1016/0006-8993(87)90557-9
  10. Reiner, A.; Northcutt, R. G. J. Comp. Neurol. 1987, 256, 463-481 https://doi.org/10.1002/cne.902560313
  11. Reiner, A.; Carraway, R. E. J. Comp. Neurol. 1987, 257, 453-476 https://doi.org/10.1002/cne.902570312
  12. De Nadai, F.; Cuber, J. C.; Kitabgi, P. Brain Res. 1989, 500, 193- 198 https://doi.org/10.1016/0006-8993(89)90313-2
  13. Carraway, R. E.; Ferris, C. F. J. Biol. Chem. 1983, 258, 2475- 2479
  14. Basir, Y. J.; Knoop, F. C.; Dulka, J.; Conlon, J. M. Biochim. Biophys. Acta 2000, 1543, 95-105 https://doi.org/10.1016/S0167-4838(00)00191-6
  15. Gilbert, J. A.; Richelson, E. Eur. J. Pharmacol. 1986, 129, 379- 383 https://doi.org/10.1016/0014-2999(86)90452-8
  16. Sumi, S.; Inoue, K.; Kogire, M.; Doi, R.; Takaori, K.; Yajima, H.; Suzuki, T.; Tobe, T. Neuropeptides 1987, 9, 247-255 https://doi.org/10.1016/0143-4179(87)90045-X
  17. Dubuc, I.; Nouel, D.; Coquerel, A.; Menard, J. F.; Kitabgi, P.; Costentin, J. Eur. J. Pharmacol. 1988, 151, 117-121 https://doi.org/10.1016/0014-2999(88)90699-1
  18. Sydbom, A.; Ware, J.; Schultz, I.; Mogard, M. H. Agents Actions 1990, 30, 146-149 https://doi.org/10.1007/BF01969023
  19. De la Fuente, M.; Garrido, J. J.; Arahuetes, R. M.; Hernanz, A. J. Neuroimmunol. 1993, 42, 97-104 https://doi.org/10.1016/0165-5728(93)90216-L
  20. Park, N. G.; Yamato, Y.; Lee, S.; Sugihara, G. Biopolymers 1995, 36, 793-801 https://doi.org/10.1002/bip.360360611
  21. Kim, C.-H.; Kim, E. J.; Go, H.-J.; Lee, H. H.; Hong, Y.-K.; Kim, H.-R.; Chung, J. K.; Park, J.-S.; Muneoka, Y.; Park, N. G. Bull. Korean Chem. Soc. 2006, 27(7), 1015-1019 https://doi.org/10.5012/bkcs.2006.27.7.1015

Cited by

  1. Isolation of a Novel Neuropeptide with Contractile Activity on the Smooth Muscle from the Snakehead Channa argus vol.45, pp.2, 2012, https://doi.org/10.5657/KFAS.2012.0114