Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Amperometric Glucose Biosensor Based on Sol-Gel-Derived Zirconia/Nafion Composite Film as Encapsulation Matrix

  • Kim, Hyun-Jung (Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University) ;
  • Yoon, Sook-Hyun (Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University) ;
  • Choi, Han-Nim (Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University) ;
  • Lyu, Young-Ku (Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University) ;
  • Lee, Won-Yong (Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University)
  • Published : 2006.01.20
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Abstract

An amperometric glucose biosensor has been developed based on the use of the nanoporous composite film of sol-gel-derived zirconia and perfluorosulfonated ionomer, Nafion, for the encapsulation of glucose oxidase (GOx) on a platinized glassy carbon electrode. Zirconium isopropoxide (ZrOPr) was used as a sol-gel precursor for the preparation of zirconia/Nafion composite film and the performance of the resulting glucose biosensor was tuned by controlling the water content in the acid-catalyzed hydrolysis of sol-gel stock solution. The presence of Nafion polymer in the sol-gel-derived zirconia in the biosensor resulted in faster response time and higher sensitivity compared to those obtained at the pure zirconia- and pure Nafion-based biosensors. Because of the nanoporous nature of the composite film, the glucose biosensor based on the zirconia/Nafion composite film can reach 95% of steady-state current less than 5 s. In addition, the biosensor responds to glucose linearly in the range of 0.03-15.08 mM with a sensitivity of 3.40 $\mu$A/mM and the detection limit of 0.037 mM (S/N = 3). Moreover, the biosensor exhibited good sensor-to-sensor reproducibility (~5%) and long-term stability (90% of its original activity retained after 4 weeks) when stored in 50 mM phosphate buffer at pH 7 at 4 ${^{\circ}C}$.

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References

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