DOI QR코드

DOI QR Code

Effect of Various Presoaking Treatments on the Microbial Contamination and Germination Ratio of Soybeans

다양한 침지처리가 대두의 미생물 오염 및 발아율에 미치는 영향

  • 김태진 (매일유업(주) 중앙연구소) ;
  • 김영진 (매일유업(주) 중앙연구소) ;
  • 정병문 (매일유업(주) 중앙연구소) ;
  • 김응률 (매일유업(주) 중앙연구소) ;
  • 최원선 (매일유업(주) 중앙연구소) ;
  • 정후길 (매일유업(주) 중앙연구소) ;
  • 전호남 (매일유업(주) 중앙연구소) ;
  • 성창현 (세종대학교 식품공학과) ;
  • 유상호 (세종대학교 식품공학과) ;
  • 김우정 (세종대학교 식품공학과)
  • Published : 2006.09.01

Abstract

The presoaking conditions of soybeans in various solution for decreasing microbial contamination and improving germination were investigated. Soybeans were treated with presoaking solutions (sterilized water, 0.1% grape fruit extract, 0.1% vitamin $B_1$ derivate solution AS5 and vitagen) at $25^{\circ}C,\;35^{\circ}C,\;45^{\circ}C\;and\;55^{\circ}C$ for $3\sim12$ hr and germinate at $25^{\circ}C$, 95%RH for 3 days. Total bacterial count and coliform count of soybeans were $6.0\times10^5CFU/g$ and $3.0\times10^5CFU/g$, respectively, and the most of presoaking treatments decreased the microbial contamination. The increase of presoaking time induces low germination ratio compared with unsoaked soybeans and the germination of soybeans was not detected at $55^{\circ}C$. The germination of soybeans was effected by presoaking solutions, temperature, and time. The optimal presoaking condition for decreasing microbial contamination and promoting initial germination was using grape fruit extract at $35^{\circ}C$ for 6 hr.

다양한 조건으로 대두를 침지하여 대두 발아시 미생물에 의한 오염을 최대한 방지하고 발아율을 높이는 조건을 검토하기 위해 대두를 멸균수, 0.1%의 자몽씨 추출물과 0.1%의 비타민 $B_1$ 유도체(AS5, vitagen)용액 등에 $25^{\circ}C,\;35^{\circ}C,\;45^{\circ}C,\;55^{\circ}C$에서 $3\sim12$시간 동안 침지시킨 후 $25^{\circ}C$, 상대습도 95%의 조건에서 3일 동안 발아시키며 발아율을 관찰하였다. 침지전 대두의 일반 미생물수는 $6.0\times10^5CFU/g$, 대장균군수는 $3.0\times10^5CFU/g$이었고, 대부분의 침지조건에서 미생물 오염 정도는 감소하였다. 침지 대두의 발아율은 침지시간이 길어질수록 미침지 대두보다 발아율이 떨어졌고 $45^{\circ}C$이상에서는 급격히 떨어져 $55^{\circ}C$에서는 발아가 거의 일어나지 않음이 관찰되었다. 대두의 발아율은 침지용액의 종류와 침지시간 및 온도에 의해 영향을 받았으며, 대두의 미생물 오염 감소와 초기발아를 유도를 위한 최적 조건은 자몽씨 추출물을 사용하여 $35^{\circ}C$에서 6시간 침지시켰을 때였다.

Keywords

References

  1. Kim WJ. 2006. Soy; health and utilization. Hyoil, Seoul. p 49-50
  2. Chu YH, Park JH, Yun SG, Kim YH, Kim SM, Jung KW. 2002. Seed characteristics of domestic breeding varieties in black soybean (Glycine max L. Merr.). Kor J Intl Agri 14: 252-259
  3. Kim JS, Kim JG, Kim WJ. 2004. Changes in isoflavone and oligosaccharides of soybeans during germination. J Food Sci Technol 36: 294-298
  4. Lee YH, Jung HO, Rhee CO. 1987. Solids loss with water uptake during soaking of soybeans. J Food Sci Technol 19: 492-498
  5. Choi HD, Kim SS, Kim SR, Lee BY. 2000. Effect of irrigating solutions on growth and rot of soybean sprouts. J Food Sci Technol 32: 1122-1127
  6. Kim SD, Kim ID, Kim MK, Lee SK, Min KS. 1997. Effect of ozonic water on the growth and putrefaction of soybean sprouts. J Food Sci Technol 9: 181-186
  7. Park WP, Cho SH, Lee DS. 1998. Effect of grapefruit seed extract and ascorbic acid on the spoilage microorganisms and keeping quality of soybean sprouts. J Kor Soc Food Sci Nutr 27: 1086-1093
  8. Choi HD, Kim SS, Kim KT, Lee JY, Park WM. 2000. Effect of presoaking treatments on growth and rot of soybean sprouts. J Food Sci Technol 32: 584-589
  9. Kim WJ, Shin ES, Kim CK, Yang CB. 1985. Factors affecting hydration rate of black soybeans. J Food Sci Technol 17: 41-44
  10. Kim DY, Suh IS, Rhee CO. 1988. Effect of temperature on the water uptake during soaking of soybeans. J Kor Agric Chem Soc 31: 46-51
  11. Cho KH, Park SK. 2005. Antibacterial effects on Bacillus Strearothermophilus by adding natural grapefruit seed extracts in soymilk. J Kor Ind Eng Chem 16: 139-143
  12. Reagor L, Gusman J, McCoy L, Carino E, Heggers JP. 2002. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: I. An in vitro agar assay. J Altern Complement Med 8: 325-332 https://doi.org/10.1089/10755530260128014
  13. Heggers JP, Cottingham J, Gusman J, Reagor L, McCoy L, Carino E, Cox R, Zhao JG. 2002. The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity. J Altern Complement Med 8: 333-340 https://doi.org/10.1089/10755530260128023
  14. Bae KG, Nam SW, Kim KN, Shin SJ, Whang YH. 2002. Water uptake and germination of soybean seed as affected by soaking condition. Kor J Crop Sci 47: 244-249

Cited by

  1. Effects of Immersion Temperatures and Times on Chestnut Fruit and Mortality of the Chestnut Weevil, Curculio sikkimensis Heller vol.53, pp.4, 2014, https://doi.org/10.5656/KSAE.2014.09.0.026