자연과학논문집 (The Journal of Natural Sciences)
- 제16권1호
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- Pages.15-29
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- 2005
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- 1225-6196(pISSN)
고온성이며 호알칼리성인 Bacillus cereus B-13으로부터 내열성, 호알카리성 Cyclodextrin Glucanotransferase의 생산과 특성
Production and Characterization of Thermo-alkalotolerant Cyclodextrin Glucanotransferase from Thermo-alkalophilic Bacillus cereus B-13
- Seo, Seung-Bo (Han Kook Shin Yak Pharm. Co., Ltd.) ;
- Kim, Jae-Ho (Bae Sang Myun Brewery Co., Ltd.) ;
- Lee, Dae-Hyong (Department of Life Science and Genetic Engineering, Paichai University) ;
- Lee, Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
- 발행 : 2005.12.30
초록
내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13 2% 가용성 전분, 1% 효모 추출물, 1%
To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isalated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1%