Journal of Animal Science and Technology

  • 제47권6호
  • /
  • Pages.1067-1074
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  • 2005
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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켄터키 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향

Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass

  • 이기원 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 김도현 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 원성혜 (경북대학교 농업과학기술연구소) ;
  • 서성 (농촌진흥청 축산연구소) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Lee, K.W. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, S.H. (Division of Applied Life Science. Gyeongsang National University) ;
  • Lee, D.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo, H.S. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, D.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, M.S. (Division of Forest Science, Gyeongsang National University) ;
  • Won, S.H. (Institute of Agri. Sci. & Tech., Kyungpook National University) ;
  • Seo, S. (National Livestock Research Institute, R.D.A.) ;
  • Lee, B.H. (Division of Applied Life Science, Gyeongsang National University)
  • 발행 : 2005.12.31
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초록

켄터키 블루그래스의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 효율적인 식물체 재분화를 위한 품종별 배양효과, 배지종류 및 배지첨가물 등의 최적조건을 조사하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 3mg/L 2,4-D와 0.1mg/L BA가 첨가된 MS배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 3mg/L BA가 첨가된 N6 배지에서 배양했을 때 57.7%의 높은 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도에는 MS 배지가, 식물체 재분화에는 N6 배지가 효과적이었다. 켄터키 블루그래스의 품종에 따른 배발생 캘러스의 유도율과 재분화 능력은 큰 차이를 나타내어 Newport가 가장 높은 배양효율을 나타내었다. 본 연구를 통하여 확립된 단기간의 효율적인 재분화시스템은 분자육종을 통한 신품종 켄터키 블루그래스 개발에 유용하게 응용되어질 수 있을 것이다.

In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.

키워드

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