International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Genetic Variability Within and Among Three Ecoraces of the Tasar Silkworm Antheraea mylitta Drury, as Revealed by ISSR and RAPD Markers

  • Vijayan K. (SeriBiotech Research Laboratory) ;
  • Nair C. V. (SeriBiotech Research Laboratory) ;
  • Kar P. K. (Central Sericultural Germplasm Resources Center) ;
  • Mohandas T. P. (SeriBiotech Research Laboratory) ;
  • Saratchandra B. (Central silk Board, BTM layout) ;
  • Urs S. Raje (SeriBiotech Research Laboratory)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Genetic diversity within and between populations of Antheraea mylitta Drury was studied using thirty individuals from three ecoraces using 12 ISSR and 10 RAPD primers. Rally, Daba and Modal ecoraces were collected from Chattisgarh, Jharkhand and Orissa states of India respectively. The ISSR and RAPD primers generated $94.7\%$ and $95.6\%$ polymorphism among the 30 individuals. The cluster analysis grouped these individuals according to their ecorace. The intra-ecoracial heterozygosity estimated with ISSR markers were $0.123{\pm}0.18,\;0.169{\pm}0.17\;and\;0.214{\pm}0.17$ respectively for Modal, Raily and Daba ecoraces. Like wise, with RAPD markers the intraecoracial heterozygosity was $0.17{\pm}0.22$ in Modal, $0.229{\pm}0.17$ in Raily and $0.23{\pm}0.19$ in Daba ecoraces. However, the significantly low genetic differentiation (GST) (0.182 for ISSR and 0.161 for RAPD) and the high gene flow (Nm) (2.249 for ISSR and 2.60 for RAPD markers) among the ecoraces revealed that the amount of genetic diversity present among the ecoraces is not significant enough to make drastic genetic drifts among these ecoraces in the near future.

Keywords

References

  1. Chatterjee, S. N., K. Vijayan, G. C. Roy and C. V. Nair (2004). ISSR profiling of genetic variability in the ecotypes of Antheraea mylitta Drury, the tropical tasar silkworm. Genetika 40, 210-217
  2. Felsenstein, J. (1993). PHYLIP (Phylogeny inference package) ver. 3.5c. Department of Genetics, University of Washington, Seattle
  3. Gepts, P. (1993) The use of molecular and biochemical markers in crop evolution studies. Evol. BioI. 27, 51-94
  4. Ghosh, C. C. (1949) Silk production and Weaving in India, CSIR Monograph, CSIR, New Delhi
  5. Jolly, M. S., S. K. Sen and M. M. Ashan (1974) Tasar culture, pp. 1-166, Ambika Publishers, Bombay
  6. Kar, P. K., A. K. Srivastava and A. H. Naqvi (2000) Changes in voltinism in some populations of Antheraea mylitta: Response to photoperiod. Int. J. Wild Silkmoth Silk 5, 176-178
  7. Mahalanobis, P. C. (1936) On the genetical distance in statistics. Proc Nat Inst. Sci. India 2, 265-275
  8. Nayak, B. K. and M. C. Dash (1991) Environmental regulation of voltinism in Antheraea mylitta Drury (Lepidoptera: Satumiidae), the Indian tasar silk insect. Sericologia 31, 479-486
  9. Nei, M. (1972) Genetic distance between population. Amer. Natur. 106, 283-292 https://doi.org/10.1086/282771
  10. Nei, M. (1973) Analysis of gene diversity in subdivided populations. Pro. Natl Acad. Sci. USA 70, 3321-3323 https://doi.org/10.1073/pnas.70.12.3321
  11. Siddiqui, A. A., A. K. Sengupta, A. Kumar, D. P. Das Mohapatra and K. Sengupta (1998) Studies on the genetic architecture and gene action in yield and yield components in diallel population of tropical tasar Antheraea mylitta D. Sericologia 28, 107-113
  12. Slatkin, M. (1987) Gene flow and geographic structure of natural populations. Science 236, 533-536
  13. Sneath, P. H. A. and R. R. Sokal (1973) Numerical taxonomy: The principles and practice of numerical taxonomy, WH Freeman &Co, San Francisco, pp. 513
  14. Srivastava, A. K., A. H. Naqvi, A. K. Sinha, S. R. Viswakarma, G. C. Roy and B. R. R. P. Sinha (2002) Biotic diversity and genetic resource conservation of tropical tasar silkworm: national imperatives; in Advances in Indian Sericulture Research. Dandin, S. B. and V. P. Gupta (eds.), pp. 387-394, Central Sericultural Research and Training Institute, Mysore, India
  15. Srivastava, A. K. A, H. Naqvi, G. C.Roy and B. R. R. P. Sinha (2000) Temporal variation in quantitative and qualitative characters of Antheraea mylitta Drury. Int. J. Wild Silkmoth Silk 5, 54-56
  16. Srivastava, A. K., P. K. Kar, A. H. Naqvi, A. K. Sinha, B. M. K. Singh, B. R. R. P. Sinha and K. Thangavelu (2001) Biochemical variation in ecoraces of Antheraea mylitta: a review. Pers. Cytol. Genet. 10, 359-364
  17. Thangavelu, K. and A. K. Sinha (1993) Population ecology of Antheraea mylitta Drury (Lepidoptera: Satumiidae). Wild Silkmoths 92, 87-92
  18. Thangavelu, K., P. K Srivastava and A. K. Srivastava (2000) Management of tropical tasar silkworm and host plant; in Proceedings of National workshop on Management of Sericultural Germplasm for Posterity, pp. 72-90, Central Sericultural Germplasm Resources Centre, Hosur, India, 26-27 July
  19. Williams, J. G K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 531-6535 https://doi.org/10.1093/nar/18.3.531
  20. Yeh, F. C.(1998) POPGENE16. Version, 1.31. (http://www.ualberta.ca/-fyeh) Agriculture and Forestry Moleclar Biology and Biotechnology center, University of Alberta and Center for International Forestry Research, Canada