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벼멸구 저항성 유전자와 내염성과의 연관

Linkage Between Brown Planthopper Resistance Gene and Salt Tolerance in Rice

  • Yang Dae Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Kim Jin-Hong (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Wi Seung Gon (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Baek Myung-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lim Sang Yong (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lee In Sok (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lee Kyu-Seong (National Institute of Crop Science, R. D. A.) ;
  • Lee Myung Chul (National Institute of Agricultural Biotechnology, R. D. A.) ;
  • Lim Yong-Pyo (Division of Plant Science and Resources, Chungnam National University) ;
  • Chung Byung Yeoup (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Kim Jae-Sung (Division of Radiation Application Research, Korea Atomic Energy Research Institute)
  • 발행 : 2005.02.01

초록

벼멸구(Nilapawata lugens Stal.)에 대한 저항성 마커 (R208)에서 확실한 차이를 보인 두 자포니카 품종(일품벼과 상해향혈나)을 대상으로 벼멸구 저항성 유전자(Os-Bil)와 내염성과의 관련성을 조사하였다. 이를 위해 두 품종에서 염처리에 의한 Os-Bil 발현량의 변화를 real-time PCR을 이용해 정량화 하였으며, 인디카 두 품종(Pokkali와 IR29)의 결과와 비교하였다. 일품벼는 50, 200 mM NaCl 처리에서 Os-Bil 유전자의 발현량이 농도 의존적으로 감소하였으며, 상해향혈나는 50mM에서만 약간 증가하고 200mM에서는 크게 감소하였다. 비교해서, 내염성인 Pokkali는 Os-Bil의 발현량이 100 mM의 NaCl 처리에 의해 약 2배 증가하였으나 감수성인 IR29는 같은 농도에서 발현량이 감소하였다. 이러한 결과들은 벼멸구 저항성 유전자(Os-Bil)가 적어도 인디카 품종들의 내염성 차이에 관여함을 의미하는 것으로 보인다.

Using two japonica rice cultivars (Ilpumbyeo and Sanghaehyanghyella), which are distinguishable by the brown planthopper (BHP) resistance maker (R208), a relationship between the BPH resistance gene (Os-Bil) and salt-tolerance was investigated. To do this, changes in the expression level of Os-Bil by the salt stress were quantified by the real-time PCR in the two cultivars, and compared with those in other two indica rice cultivars (Pokkali and IR29). In Ilpumbyeo, the expression level of Os-Bil decreased by the treatments of 50 and 200 mM NaCl in a concentration-dependent manner, and in Sanghaehyanghyella it rather increased slightly at 50 mM but decreased drastically at 200 mM. Comparably, IR29, a salt-sensitive cultivar, showed a reduction of the Os-Bil gene expression after the treatment of 100 mM NaCl, but Pokkali, a salt-tolerance cultivar, rather increased about two times in the level of Os-Bil transcripts. These results suggest that the BPH resistance gene may involve in the difference in the salt-tolerance at least between the two indica rice cultivars.

키워드

참고문헌

  1. Ahn, S.N., Y.K. Kim, H.C Hong, S.S. Han,S.J. Kwon, H.C. Choi, H.P. Moon and S.R. McCouch. 2000. Molecular mapping of a new gene for resistance to rice blast (Pyricularia gresea Sac.). Euphytica 116, 17-22 https://doi.org/10.1023/A:1004045812357
  2. Akbar, M. and T. Yabuno. 1975. Breeding for salin-resistant varieties of rice, III Response of F1 hybrids to salinity in reciprocal crosses between Jhona 349 and Magnolia. Jpn. J. Breed. 25, 215-220 https://doi.org/10.1270/jsbbs1951.25.215
  3. Akbar, M. and T. Yabuno. 1977. Breeding for salin-resistant varieties of rice, IV Inheritance of delayed-type panicle sterility induced by salinity. Jpn. J. Breed. 27, 237-240 https://doi.org/10.1270/jsbbs1951.27.237
  4. Baek, M.H., J.H. Kim, BY Chung, J.S. Kim and I.S. Lee. 2004. Alleviation of salt stress by low dose T-irradiation in rice (Oryza sativa L.). Biol. Plant (in press)
  5. Brar, D.S. and G.S. Khush. 1997. Alien introgression in rice. Plant Mol. Biol. 35, 35-47 https://doi.org/10.1023/A:1005825519998
  6. Cho, C.I., D.H. Kim and J.H. Yuh. 1988. Resistance aspects of tongil and japonica varieties to brown planthopper (Nilaparvata lugens Sta1.). Res. Rept. RDA (R) 30, 1-6
  7. Crace, M.B., C.B. Mcleland and W.F. Blakely. 2002. Realtime quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry. Int. J. Radiat. Biol. 78, 1011-1021 https://doi.org/10.1080/09553000210158056
  8. Ha, W.H., K.M. Kim and J.K. Sohn. 1999. Detection of resistance gene to brwon planthopper (Nilaparvata lugens Stal.) using RFLP and Isozyme marker in rice. Kor. J. Breed. 32, 319-322
  9. Heid, C.A., J. Stevens. J.K. Livak and P.M. Williams. 1996. Real time quantitative PCR. Genome Res. 6, 986-994 https://doi.org/10.1101/gr.6.10.986
  10. Hirabayashi, H. and O. Tsugufumi. 1995. RFLP mapping of Bph 1 (Brown Planthopper Resistance Gene) in rice. Breed. Sci. 45, 369-371
  11. Huang, N., A. Parco, T. Mew, G. Magpantay, S. McCouch, E. Guiderdoni, J. Xu, P. Subudhi, E.R. Angeles and G.S. Khush. 1997. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Mol. Breed. 2, 105-113
  12. Jeon, Y.H, S.N. Ahn, H.C. Choi, T.R. Hahn and H.P. Moon 1999. Identification of a RAPD marker linked to a brown planthopper resistance gene in rice. Euphytica 107, 23-28 https://doi.org/10.1023/A:1003506830735
  13. Ji, H.S., H.J. Koh, S.U. Park and S.R. McCouch. 1998. Varietal identification in japonica rice using microsatellite DNA markers. Kor. J. Breed. 30, 350-360
  14. Kaneda, C. and R. Kisimoto. 1979. Status of varietal resistance to brown planthopper in Japan. In: brown planthopper-threat to rice production in asia, pp 209-218, IRRI. Los Banos. Philippines
  15. Kaneda, C. 1984. Studies on breeding japonica rice resistant to the brown planthopper (Nilaparvata lugens Stal.). Bull. Nat. Agric. Res. Cent. 2, 1-74
  16. Kim, S.M., S.J. Yang and J.K. Sohn. 2003. Development of a DNA marker for selection of resistanct plants to brown planthopper (BPH) in rice. Kor. J. Breed. 35, 277-282
  17. Kwon, S.J., S.N. Ahn, J.P. Suh, H.C. Hong, Y.K. Kim, H.G. Hwang, H.P. Moon and H.C. Choi. 2000. Genetic diversity of Korean native rice varieties. Kor. J. Breed. 32, 186-193
  18. Mohler, V., A. Klahr, G. Wenzel and G. Schwarz. 2002. A resistance gene analog useful for targeting disease resistance genes against different pathogens on group IS chromosomes of barley, wheat and rye. Theor. Appl. Genet. 105, 364-368 https://doi.org/10.1007/s00122-002-0919-z
  19. Murai, H., Z. Hashimoto, P.N. Sharma, T. Shimizu, K. Murata, S. Takumi, N. Mori, S. Kawasaki and C. Nakamura. 2001. Construction of high resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stal.) resistance gene bph 2. Theor. Appl. Genet. 103, 526-532 https://doi.org/10.1007/s001220100598
  20. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  21. Olufowote, J.O., Y. Xu, X. Chen, W.D. Park, H.M. Beachell, R.H. Dilday, M Goto and S.R. McCouch. 1997. Comparative evaluation of within-cultivar variation of rice (Oryza sativa L.) using microsatellite and RFLP markers. Genome 40, 370-378 https://doi.org/10.1139/g97-050
  22. Sardesai, N., A. Kuma, K.R. Rajyashri, S. Nair and M. Mohan. 2002. Identification and mapping of an AFLP marker linked to Gm7, a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice. Theor. Appl. Genet. 105, 691-698 https://doi.org/10.1007/s00122-002-1035-9
  23. Shin, M.S. 1990. Genetic analysis for resistance to bacterial blight and brown planthopper in japonica rice cultivars breed in Korea. pp 1-53, Ph. D. Thesis. Chonnam Nat. Univ., Chonnam
  24. Xie, J.H., F.F. Zapata-Arias, M. Shen and R. Afza. 2000. Salinity tolerant performance and genetic diversity of four rice varieties. Euphytica 116, 105-110 https://doi.org/10.1023/A:1004041900101
  25. Xu, X.F., H. Mei, W. Luo, X.N. Cheng and Z.K. Li. 2002. RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens Stal.). Theor. Appl. Genet. 104, 248-253 https://doi.org/10.1007/s00122-001-0777-0
  26. Yamanouchi, U., M. Yano, H. Lin, M. Ashikari and K. Yamada. 2002. A rice spotted leaf gene, Sp17, encodes a heat stress transcription factor protein. Proc. Natl. Acad. Sci. 99, 7530-7535
  27. Yeo, U.S. and J.K. Sohn. 2001. Linkage analysis between some agroanomic traits and resistance gene to brown planthopper in rice. Kor. J. Breed. 33, 287-293