Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Linkage Between Brown Planthopper Resistance Gene and Salt Tolerance in Rice

벼멸구 저항성 유전자와 내염성과의 연관

  • Yang Dae Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Kim Jin-Hong (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Wi Seung Gon (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Baek Myung-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lim Sang Yong (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lee In Sok (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Lee Kyu-Seong (National Institute of Crop Science, R. D. A.) ;
  • Lee Myung Chul (National Institute of Agricultural Biotechnology, R. D. A.) ;
  • Lim Yong-Pyo (Division of Plant Science and Resources, Chungnam National University) ;
  • Chung Byung Yeoup (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Kim Jae-Sung (Division of Radiation Application Research, Korea Atomic Energy Research Institute)
  • Published : 2005.02.01
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Abstract

Using two japonica rice cultivars (Ilpumbyeo and Sanghaehyanghyella), which are distinguishable by the brown planthopper (BHP) resistance maker (R208), a relationship between the BPH resistance gene (Os-Bil) and salt-tolerance was investigated. To do this, changes in the expression level of Os-Bil by the salt stress were quantified by the real-time PCR in the two cultivars, and compared with those in other two indica rice cultivars (Pokkali and IR29). In Ilpumbyeo, the expression level of Os-Bil decreased by the treatments of 50 and 200 mM NaCl in a concentration-dependent manner, and in Sanghaehyanghyella it rather increased slightly at 50 mM but decreased drastically at 200 mM. Comparably, IR29, a salt-sensitive cultivar, showed a reduction of the Os-Bil gene expression after the treatment of 100 mM NaCl, but Pokkali, a salt-tolerance cultivar, rather increased about two times in the level of Os-Bil transcripts. These results suggest that the BPH resistance gene may involve in the difference in the salt-tolerance at least between the two indica rice cultivars.

벼멸구(Nilapawata lugens Stal.)에 대한 저항성 마커 (R208)에서 확실한 차이를 보인 두 자포니카 품종(일품벼과 상해향혈나)을 대상으로 벼멸구 저항성 유전자(Os-Bil)와 내염성과의 관련성을 조사하였다. 이를 위해 두 품종에서 염처리에 의한 Os-Bil 발현량의 변화를 real-time PCR을 이용해 정량화 하였으며, 인디카 두 품종(Pokkali와 IR29)의 결과와 비교하였다. 일품벼는 50, 200 mM NaCl 처리에서 Os-Bil 유전자의 발현량이 농도 의존적으로 감소하였으며, 상해향혈나는 50mM에서만 약간 증가하고 200mM에서는 크게 감소하였다. 비교해서, 내염성인 Pokkali는 Os-Bil의 발현량이 100 mM의 NaCl 처리에 의해 약 2배 증가하였으나 감수성인 IR29는 같은 농도에서 발현량이 감소하였다. 이러한 결과들은 벼멸구 저항성 유전자(Os-Bil)가 적어도 인디카 품종들의 내염성 차이에 관여함을 의미하는 것으로 보인다.

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