Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Subacute Inhalation Toxicity Study of Dimethyl Disulfide in F344 Rats

F344 랫드를 이용한 이황화메틸의 아급성 흡입독성연구

  • Kim Hyeon-Yeong (Industrial Chemicals Research Center, Industrial Safety and Health Research Institute, Korea Industrial Safety Corporation) ;
  • Lee Sung-Bae (Industrial Chemicals Research Center, Industrial Safety and Health Research Institute, Korea Industrial Safety Corporation) ;
  • Han Jung-Hee (Industrial Chemicals Research Center, Industrial Safety and Health Research Institute, Korea Industrial Safety Corporation) ;
  • Chung Yong-Hyun (Industrial Chemicals Research Center, Industrial Safety and Health Research Institute, Korea Industrial Safety Corporation) ;
  • Kim Hyoung-Chin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin Jin-Young (College of Veterinary Medicine, Chonnam National University) ;
  • Shin Dong-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee Young-Mook (Industrial Chemicals Research Center, Industrial Safety and Health Research Institute, Korea Industrial Safety Corporation)
  • 김현영 (산업안전보건연구원 산업화학물질연구센터) ;
  • 이성배 (산업안전보건연구원 산업화학물질연구센터) ;
  • 한정희 (산업안전보건연구원 산업화학물질연구센터) ;
  • 정용현 (산업안전보건연구원 산업화학물질연구센터) ;
  • 김형진 (한국생명공학연구원) ;
  • 신진영 (전남대학교 수의과대학) ;
  • 신동호 (전남대학교 수의과대학) ;
  • 김종춘 (전남대학교 수의과대학) ;
  • 이용묵 (산업안전보건연구원 산업화학물질연구센터)
  • Published : 2005.02.01
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Abstract

The purpose of this study was to investigate the potential subacute toxicity of dimethyl disulfide by 3 weeks inhalation in F344 rats. The test article, dimethyl disulfide was exposed by inhalation to male and female rats at dose levels of 0, 5, 25, or 125 ppm/6 hrs/ day for 3 weeks. Five rats/ sex/ group were sacrificed on day 4 after the initiation of treatment, while 5 rats/ sex/ group were sacrificed at the end of treatment period. During the test period, clinical signs, mortality, body weights, food consumption, hematology, serum biochemistry, and gross findings were examined. Slight decreases in body weight gain were noted in both sexes of the highest dose group in a dose dependent manner but were only statistically different from the control animals in males of the group. A slight non-significant reduction in food consumption were also noted in the both sexes of the highest dose group. There were no adverse effects on mortality, clinical signs, hematology, serum biochemistry, and necropsy findings at any dose tested. Based on these results, it was concluded that the 3 weeks repeated dose of dimethyl disulfide by inhalation resulted in suppressed body weight gain and decreased food consumption at 125 ppm of both sexes. In the present experimental conditions, the target organ was not determined in rats. The no­observed-adverse-effect level (NOAEL) was considered to be 25 ppm/6 hrs/day for both sexes.

이황화메틸의 반복 흡입노출에 의한 아급성 독성 잠재력을 평가하기 위해 암수 랫드에게 0, 5, 25 및 125 ppm용량으로 21일간 반복 흡입노출하고, 일반증상과 체중, 사료섭취 량, 혈액치, 혈청생화학치 및 부검소견을 관찰하였다. 시험결과, 랫드에게 이황화메틸을 3주간 반복 흡입노출하면 125 ppm의 농도에서 체중증가의 억제와 사료섭취 량의 감소를 유발하나, 혈액 및 혈청생화학치에는 어떠한 이상도 유발하지 않는 것으로 나타났다. 본 시험 조건 하에서 이황화메틸의 표적 장기는 관찰되지 않았으며, 무해용량은 암수 모두 25 ppm으로 사료된다.

Keywords

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