Journal of Korea Technical Association of The Pulp and Paper Industry (펄프종이기술)

Korea Technical Association of the Pulp and Paper Industry (한국펄프종이공학회)
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Screening of Microorganisms Secreted High Efficient Enzymes and Properties of Enzymatic Deinking for Old Newsprint(IV) -Culture conditions of fungal extracellular enzyme production for biological deinking system-

고효율 효소를 분비하는 균주의 선발 및 신문고지의 효소탈묵 특성(제4보) -고지탈묵용 Fungal Cellulase와 Xylanase의 생산-

  • Park Seong-Cheol (Division of Forest Science, College of Agriculture and Life Sciences, Chonbuk National University) ;
  • Kang Jin-Ha (Division of Forest Science, College of Agriculture and Life Sciences, Chonbuk National University) ;
  • Lee Yang-Soo (Division of Forest Science, College of Agriculture and Life Sciences, Chonbuk National University)
  • 박성철 (전북대학교 농업생명과학대학 산림과학부) ;
  • 강진하 (전북대학교 농업생명과학대학 산림과학부) ;
  • 이양수 (전북대학교 농업생명과학대학 산림과학부)
  • Published : 2005.03.01
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Abstract

This study was focused on the optimum culture condition in CMCase, FPase and xylanase activities of two fungal strains that secret extracellular enzymes for using enzymatic deinking agent to old newsprint. The results of this study were as follows. When Fusarium pallidoroseum was grown on the medium, containing of rice bran+xylan $2.0\%,\;peptone\;0.6\%,\;KH_2PO_4\;0.075\%\;and\;MnSO_4\;0.06\%\;with\;pH\;9.0,\;at\;29^{\circ}C$ for 6 days, the quantitative degree of extracellular enzyme production was the highest. Optimum culture condition for Aspergillus niger was pH 5.0, $27^{\circ}C$ incubating temperature and 7 days incubation period on liquid medium, containing of CMC+xylan $2.5\%,\;yeast\;extract\;0.4\%,\;K_3PO_4\;0.05\%\;and\;CaCl_2+FeSO_4\;0.08\%$. Aspergillus niger was fairly higher FPase and xylanase activities than Trichoderma reesei ATCC 28217.

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References

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