Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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A spsB Gene Putatively Encoding Glucosyl-Isopreny Phosphate-Transferase in Sphingomonas chungbukensis DJ77

Sphingomonas chungbukensis DJ77의 Glucosyl-Isoprenyl Phosphate-Transferase를 암호화할 것으로 추정되는 spsB 유런자

  • Lee Soo-Youn (School of Life Science, Chungbuk National University) ;
  • Choi Jung-Do (School of Life Science, Chungbuk National University) ;
  • Shin Malshick (Department of Food and Nutrition, Chonnam National University) ;
  • Kim Young-Chang (School of Life Science, Chungbuk National University, Biotechnology Research Institute, Chungbuk National University)
  • 이수연 (충북대학교 자연과학대학 생명과학부) ;
  • 최정도 (충북대학교 자연과학대학 생명과학부) ;
  • 신말식 (전남대학교 생활과학대학 식품영양학과) ;
  • 김영창 (충북대학교 자연과학대학 생명과학부, 충북대 바이오 연구소)
  • Published : 2005.03.01
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Abstract

Some genes, which are involved in the biosynthesis of polysaccharides, could be found by the genome project of Sphingomonas chungbukensis DJ77. In this study, we identified the complete nucleotide sequence of a gene, encoding the glucosyl-isoprenyl phosphate-transferase, which catalyzes the first step in the biochemical pathway for the synthesis of the sphingan type polysaccharide. This gene, named spsB, is initiated by the ATG codon and terminated by the TGA, and its open reading frame consists of 1392 bp, encoding 463 amino acids. The predicted amino acid sequence of this enzyme indicates $50\%$ similarity to SpsB of Sphingomonas spp S88, also produces sphingan, and $48\%$ to GelB of Sphingomonas paucimobilis ATCC 31461.

S. chungbukensis DJ77의 genome project수행 결과 다당류 생 합성 에 관련된 유전자들의 염기서열을 찾아내었다. 본 논문에서는 이러한 유전자들 중 sphigan형 다당류 생합성에 관여하는 glucosyl-isoprenyl phosphate-transferase를 암호화 하는 유전자의 완전한 서열을 결정하였고, spsB로 명명하였다. 이 유전자는 ATG를 개시코돈으로 사용하며, TGA를 종결코돈으로 사용하고 있다. 또한 총 1392 bp의 open reading frame을 포함하며, 463개의 아미노산으로 구성되어있다. SpsB를 구성하는 아미노산 서열은 동일한 속의 sphingan 형성 균주인 Sphingomonas spp S88의 SpsB와 $50\%$, Sphingomonas paucimobilis ATCC 31461의 GelB와 $48\%$의 유사성을 나타내었다.

Keywords

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