Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Purification of Vasopressin-related Peptide, [Arg8]-vasotocin, from the Brain of Conger Eel (Conger myriaster)

붕장어 (Conger myriaster)의 뇌로부터 Vasopressin-related Peptide, [Arg8]-vasotocin의 정제

  • Go Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM In Hye (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Hee Yeon (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • HONG Yong-Ki (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 박희연 (국립수산과학원 생명공학연구단) ;
  • 윤호동 (국립수산과학원 생명공학연구단) ;
  • 홍용기 (부경대학교 수산과학대학 생물공학과) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • Published : 2005.02.01
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Abstract

Vasopressin (VP)-related peptide was purified from the brain extract of conger eel (Conger myriaster) by reverse-phase, ion-exchange high performance liquid chromatography (HPLC). This peptide with a molecular weight of 1,051.2 Da was determined as $H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Arg-Gly-NH_2$, whose Cys residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis, MALDI- TOF mass spectrometry. It's sequence was confirmed by identity of the elution position with the synthetic peptide in HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of VP-superfamily member, $[Arg^8]-vasotocin$. The synthetic peptide showed a contractile activity at a minimal effective concentration of $10^{-10}\;M$ on the intestinal smooth muscle of goldfish.

Keywords

References

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