Parasites, Hosts and Diseases

  • 제43권1호
  • /
  • Pages.33-37
  • /
  • 2005
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지

Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease

  • SHIN Myeong Heon (Department of Parasitology, Institute of Tropical Medicine, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • CHUNG Young-Bae (Department of Parasitology, College of Medicine, Cheju National University) ;
  • KITA Hirohito (Department of Internal Medicine and Immunology, Mayo clinic and Mayo Foundation, Rochester)
  • 발행 : 2005.03.01
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초록

Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EON) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.

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참고문헌

  1. Abu-Ghazaleh RI, Fujisawa T, Mestecky J, Kyle RA, Gleich GJ (1989) IgA-induced eosinophil degranulation. J Immunol 142: 2393-2400
  2. Asokananthan N, Graham PT, Stewart DJ, Bakker AJ, Eidne KA, Thompson PI, Stewart GA (2002) House dust mite allergens induce proinflammatory cytokines from respiratory epithelial cells: the cysteine protease allergen, Der p 1, activates protease-activated receptor (PAR)-2 and inactivates PAR-l. J Immunol 169: 4572-4578 https://doi.org/10.4049/jimmunol.169.8.4572
  3. Chung YB, Kong Y, Joo IJ, Cho SY, Kang SY (1995) Excystment of Paragonimus westermani metacercariae by endogenous cysteine protease. J Parasitol 81: 137-142 https://doi.org/10.2307/3283911
  4. Chung YB, Kong Y, Yang HJ, Kang SY, Cho SY (1997a) Cysteine protease activities during maturation stages of Paragonimus westermani. J Parasitol 83: 902-907 https://doi.org/10.2307/3284286
  5. Chung YB, Yang HJ, Kang SY, Kong Y, Cho SY (1997b) Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG. Korean J Parasitol. 35: 139-142 https://doi.org/10.3347/kjp.1997.35.2.139
  6. Dery O, Corvera CU, Steinhoff M, Bunnett NW (1998) Protease-activated receptors: novel mechanisms of signaling by serine proteases. Am J Physiol 274: C1429-C1452
  7. Gleich GJ, Adolphson CR (1996) The eosinophilic leukocytes: structure and function. Adv Immunol 39: 177-253
  8. Horie S, Gleich GJ, Kita H (1996) Cytokines directly induce degranulation and superoxide production from human eosinophils. J Allergy Clin Immunol 98: 371-381 https://doi.org/10.1016/S0091-6749(96)70161-6
  9. Ishii K, Hein L, Kobilka B, Coughlin SR (1993) Kinetics of thrombin receptor cleavage on intact cells. Relation to signaling. J Biol Chem 268: 9780-9786
  10. Kauffman HF, Tomee JF, van de Riet MA, Timmerman AJ, Borger P (2000) Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production. J Allergy Clin Immunol 105: 1185-1193 https://doi.org/10.1067/mai.2000.106210
  11. King C, Brennan S, Thompson PJ, Stewart GA (1998) Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol 161: 3645-3651
  12. Kita H, Adophlson CR, Gleich GJ (1998). Biology of eosinophils. In Allergy: Principles & Practice, Vol. 1, 5th ed. E. Middleton, Jr., C.E. Reed, E.F. Ellis, N.F. Adkinson, Jr. J.W. Yunginger, and W.W. Busse, eds. Mosby; St. Louis, pp. 242-260
  13. Kroegel C, Yukawa T, Dent G, Venge P, Chung KF, Barnes PI (1989) Stimulation of degranulation from human eosinophils by platelet activating factor. J Immunol 142: 3518-3526
  14. Lourbakos A, Potempa J, Travis J, D'Andrea MR, Andrade-Gordon P, Santulli R, Mackie EJ, Pike RN (2001a) Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion. Infect Immun 69: 5121-5130 https://doi.org/10.1128/IAI.69.8.5121-5130.2001
  15. Lourbakos A, Yuan YP, Jenkins AL, Travis J, Andrade-Gordon P, Santulli R, Potempa J, Pike RN (2001b) Activation of protease-activated receptors by gingipains from Porphyromonas gingivalis leads to platelet aggregation: a new trait in microbial pathogenecity. Blood 97: 3790-3797 https://doi.org/10.1182/blood.V97.12.3790
  16. Macfarlane SR, Seatter MJ, Kanke T, Hunter GD, Plevin R (2001) Proteinase-activated receptors. Pharmacol Rev 53: 245-282
  17. Miike S, Kita H (2003) Human eosinophils are activated by cysteine proteases and release inflammatory mediators. J Allergy Clin Immunol 111: 704-713 https://doi.org/10.1067/mai.2003.1332
  18. Miike S, McWilliam AS, Kita H (2001) Trypsin induces activation and inflammatory mediator release from human eosinophils through protease-activated receptor-2. J Immunol 167: 6615-6622 https://doi.org/10.4049/jimmunol.167.11.6615
  19. Racz P, Tenner-Racz K, Buttner DW, Albiez EJ (1982) Ultrastructural evidence for eosinophil-parasite adherence (EPA) reaction in human onchocercal lymphadenitis in the early period following diethylcarbamazine treatment. Trop Med Parasitol 33: 213-218
  20. Shin MH, Kita H, Park HY, Seoh JY (2001) Cysteine protease secreted by Paragonimus westermani attenuates effector functions of human eosinophils stimulated with immunoglobulin G. Infect Immun 69: 1599-1604 https://doi.org/10.1128/IAI.69.3.1599-1604.2001
  21. Shin MH, Lee SY (2000) Proteolytic activity of cysteine protease in excretory-secretory product of Paragonimus westermani newly excysted meta cercariae pivotally regulates IL-8 production of human eosinophils. Parasite Immunol 22: 529-533 https://doi.org/10.1046/j.1365-3024.2000.00337.x
  22. Sun G, Stacey MA, Schmidt M, Mori L, Mattoli S (2001) Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells. J Immunol 167: 1014-1021 https://doi.org/10.4049/jimmunol.167.2.1014
  23. Zeck-Kapp G, Kroegel C, Riede UN, Kapp A (1995) Mechanisms of human eosinophil activation by complement protein C5a and platelet-activating factor: similar functional responses are accompanied by different morphologic alterations. Allergy 50: 34-47