Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Identification of Inducible Genes during Mast Cell Differentiation

  • Published : 2005.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Mast cells play an important role in allergic inflammation by releasing their bioactive mediators. The function of mast cells is enhanced by stimulation because of the induction of specific genes and their products. While many inducible genes have been elucidated, we speculated that a significant number of genes remain to be identified. Thus, we applied differential display (dd) PCR to establish a profile of the induced genes in bone marrow-derived mast cells (BMMCs) after they were co-cultured with 3T3 fibroblasts. To date, 150 cDNA fragments from the connective-type mast cells (CTMCs) were amplified. Among them, thirty cDNA fragments were reamplified for cloning and sequencing. The ddPCR strategy revealed that serine proteases were the most abundant genes among the sequenced clones induced during the maturation. Additionally, unknown genes from the co-culture of BMMCs with 3T3 fibroblasts were identified. We confirmed their induction in the CTMCs by Northern blot analysis and RT-PCR. Characterization of these induced genes during the maturation processes will provide insight into the functions of mast cells.

Keywords

References

  1. Beaven, M. A. and Baumgartner, R. A., Downstream signals initiated in mast cells by Fc epsilon RI and other receptors. Curr. Opin. Immunol., 8, 766-772 (1996) https://doi.org/10.1016/S0952-7915(96)80002-1
  2. Darmon, A. J., Nicholson, D. W., and Bleackley, R. C., Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B. Nature, 377, 446-448 (1995) https://doi.org/10.1038/377446a0
  3. Dayton, E. T., Pharr, P., Ogawa, M., Serafin, W. E., Austen, K. F., Levi-Schaffer, F., and Stevens, R. L., 3T3 fibroblasts induce cloned interleukin 3-dependent mouse mast cells to resemble connective tissue mast cells in granular constituency. Proc. Natl. Acad. Sci. U.S.A., 85, 569-572 (1988) https://doi.org/10.1073/pnas.85.2.569
  4. Echtenacher, B., Mannel, D. N., and Hultner, L., Critical protective role of mast cells in a model of acute septic peritonitis. Nature, 381, 75-77 (1996) https://doi.org/10.1038/381075a0
  5. Friend, D. S., Ghildyal, N., Austen, K. F., Gurish, M. F., Matsumoto, R., and Stevens, R. L., Mast cells that reside at different locations in the jejunum of mice infected with Trichinella spiralis exhibit sequential changes in their granule ultrastructure and chymase phenotype. J. Cell Biol., 135, 279-290 (1996) https://doi.org/10.1083/jcb.135.1.279
  6. Galli, S. J., Tsai, M., and Lantz, C. S., In Signal transduction in mast cells and basophils(Eds, Razin, E., and Rivera, J.) Springer-Verlag, New York, NY, 11 (1999)
  7. Galli, S. J. and Wershil, B. K., The two faces of the mast cell [news; comment]. Nature, 381, 21-22 (1996) https://doi.org/10.1038/381021a0
  8. Kikuchi-Yanoshita, R., Taketomi, Y., Koga, K., Sugiki, T., Atsumi, Y., Saito, T., Ishii, S., Hisada, M., Suzuki-Nishimura, T., Uchida, M. K., Moon, T. C., Chang, H. W., Sawada, M., Inagaki, N., Nagai, H., Murakami, M., and Kudo, I., Induction of PYPAF1 during in vitro maturation of mouse mast cells. J. Biochem. (Tokyo), 134, 699-709 (2003) https://doi.org/10.1093/jb/mvg195
  9. Kitamura, Y., Morii, E., and Jippo, T., In Signal transduction in mast cells and basophils (Eds, Razin, E., and Rivera, J.) Springer-Verlag, New York, NY, 31 (1999)
  10. Lantz, C. S. and Huff, T. F., Differential responsiveness of purified mouse c-kit+ mast cells and their progenitors to IL-3 and stem cell factor. J. Immunol., 155, 4024-4029 (1995)
  11. Levi-Schaffer, F., Austen, K. F., Gravallese, P. M., and Stevens, R. L., Coculture of interleukin 3-dependent mouse mast cells with fibroblasts results in a phenotypic change of the mast cells. Proc. Natl. Acad. Sci. ., 83, 6485-6488 (1986) https://doi.org/10.1073/pnas.83.17.6485
  12. Levi-Schaffer, F., Dayton, E. T., Austen, K. F., Hein, A., Caulfield, J. P., Gravallese, P. M., Liu, F. T., and Stevens, R. L., Mouse bone marrow-derived mast cells cocultured with fibroblasts. Morphology and stimulation-induced release of histamine, leukotriene B4, leukotriene C4, and prostaglandin D2. J. Immunol., 139, 3431-3441 (1987)
  13. Liang, P. and Pardee, A. B., Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 257, 967-971 (1992) https://doi.org/10.1126/science.1354393
  14. Malaviya, R., Ikeda, T., Ross, E., and Abraham, S. N., Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha. Nature, 381, 77-80 (1996) https://doi.org/10.1038/381077a0
  15. Nakano, T., Sonoda, T., Hayashi, C., Yamatodani, A., Kanayama, Y., Yamamura, T., Asai, H., Yonezawa, T., Kitamura, Y., and Galli, S. J., Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells. J. Exp. Med., 162, 1025-1043 (1985) https://doi.org/10.1084/jem.162.3.1025
  16. Ogasawara, T., Murakami, M., Suzuki-Nishimura, T., Uchida, M. K., and Kudo, I., Mouse bone marrow-derived mast cells undergo exocytosis, prostanoid generation, and cytokine expression in response to G protein-activating polybasic compounds after coculture with fibroblasts in the presence of c-kit ligand. J. Immunol., 158, 393-404 (1997)
  17. Sambrook, J., Fritsch, E., and Maniatis, T., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, NY, (1989)
  18. Stevens, R. L. and Austen, K. F., Recent advances in the cellular and molecular biology of mast cells. Immunol. Today, 10, 381-386 (1989) https://doi.org/10.1016/0167-5699(89)90272-7
  19. Swieter, M., Midura, R. J., Nishikata, H., Oliver, C., Berenstein, E. H., Mergenhagen, S. E., Hascall, V. C., and Siraganian, R. P., Mouse 3T3 fibroblasts induce rat basophilic leukemia (RBL-2H3) cells to acquire responsiveness to compound 48/ 80. J. Immunol., 150, 617-624 (1993)
  20. Taketomi, Y., Sugiki, T., Saito, T., Ishii, S., Hisada, M., Suzuki- Nishimura, T., Uchida, M. K., Moon, T. C., Chang, H. W., Natori, Y., Miyazawa, S., Kikuchi-Yanoshita, R., Murakami, M., and Kudo, I., Identification of NDRG1 as an early inducible gene during in vitro maturation of cultured mast cells. Biochem. Biophys. Res. Commun., 306, 339-346 (2003) https://doi.org/10.1016/S0006-291X(03)00942-2
  21. Tsuji, K., Nakahata, T., Takagi, M., Kobayashi, T., Ishiguro, A., Kikuchi, T., Naganuma, K., Koike, K., Miyajima, A., and Arai, K., et al., Effects of interleukin-3 and interleukin-4 on the development of 'connective tissue-type' mast cells: interleukin-3 supports their survival and interleukin-4 triggers and supports their proliferation synergistically with interleukin 3. Blood, 75, 421-427 (1990)
  22. Tsuji, K., Zsebo, K. M., and Ogawa, M., Murine mast cell colony formation supported by IL-3, IL-4, and recombinant rat stem cell factor, ligand for c-kit. J. Cell Physiol., 148, 362-369 (1991) https://doi.org/10.1002/jcp.1041480306
  23. Wong, G., Friend, D. S., and Stevens, R. L., In Signal transduction in mast cells and basophils (Eds, Razin, E., and Rivera, J.) Springer-Verlag, New York, NY, 39 (1999)