YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Alternation in the Cell-mediated Immune Reaction by Bisphenol A Exposure in vitro

In vitro에서 Bisphenol A 노출에 의한 세포성 면역반응의 변화

  • 표명윤 (숙명여자대학교 약학대학)
  • Published : 2005.02.01
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Abstract

In order to investigate the effects of bisphenol A (BPA) on cell mediated immune reaction in vitro we examined the allogenic mixed lymphocyte reaction (MLR), splenocytes proliferation (SP) to T cell mitogens and IFN-${\gamma}\;production$. Splenocytes of Balb/c mice ($1.5{\times}10^5$ cells/well) were co-cultured with different numbers of mitomycin C-treated mature dentritic cells (DCs) in presence of BPA (25, 50, 100 ${\mu}M$) and $[^{3}H]$thymidine incorporation (cpm) was measured by scintilation counting. Splenocytes ($2{\times}10^6$ cells/well) were cultured with mitogens, Con A ($2\;{\mu}g/ml$), PHA ($5\;{\mu}g/ml$) and IL-2 ($0.1\;{\mu}g/ml$), or PMA ($5\;{\mu}g/ml$) and INO ($1\;{\mu}g/ml$) in presence of BPA (1, 10, 25, 50, 100 ${\mu}M$) and SP was assessed by MTT assay. $IFN-{\gamma}$ levels in culture supernant were determined by ELISA. At low concentration, BPA slightly increased MLR, SP and $IFN-{\gamma}$ levels, but at higher concentration it showed significant inhibitory effects on these immunological parameters. These results indicate that BPA is able to alternate cell-mediated immune reaction.

Keywords

References

  1. Krishnan, A V., Stathis, P., Permuth, S. F., Tokes, L. and Feldman, D. : Bisphenol-A: An estrogenic substance is released from polycarbonate flasks during autoclaving. Endo. 132, 2279 (1993) https://doi.org/10.1210/en.132.6.2279
  2. Mountfort, K. A., Kelly, J., Jickells, S. M. and Castle, L. : Investigations into the potential degradation of polycarbonate baby bottles during sterilization with consequent release of bisphenol A. Food Addit Contam. 14, 737 (1997) https://doi.org/10.1080/02652039709374584
  3. Goodson, A., Summerfield, W. and Cooper, I. : Survey of bisphenol A and bisphenol F in canned foods. Food Addit Contam. 19, 796 (2002) https://doi.org/10.1080/02652030110071327
  4. Sakurai, H., Maeda, M., Miyahara, K., Nakayama, M., Murayama, H., Hasegawa, H., Hayakawa, M., Sugiura, T. and Kamikura, K. : Extraction of bisphenol-A from a cardiopulmonary bypass circuit. Kyobu Geka 55, 770 (2002)
  5. Olea, N., Pulgar, R,, Perez, P., Olea-Serrano, F., Rivas, A., Novillo-Fertrell, A., Pedreaza, V., Soto, A M. and Sonnenschein, C. : Estrogenecity of resin-based composites and sealants used in dentistry. Environmental Health Perspectives 104, 298 (1996) https://doi.org/10.2307/3432888
  6. Kang, J. and Kondo, F. : Determination of bisphenol A in canned pet foods. Res. Vet. Sci. 73, 177 (2002) https://doi.org/10.1016/S0034-5288(02)00102-9
  7. Chen, M. Y., Ike, M. and Fujita, M. : Acute toxicity, mutagenicity, and estrogenecity of bisphenol-A and other bisphenols. Environ. Toxicol. 17, 80 (2002) https://doi.org/10.1002/tox.10035
  8. Papaconstantinou, A. D., Umbreit, T. H., Fisher, B. R., Goering, P. L., Lappas, N. T. and Brown, K. M. : Bisphenol A-induced increase in uterine weight and alteration in uterine morphology in ovariectomized B6C3F1 mice: role of the estrogen receptor. Toxicol Sci. 56, 332 (2000) https://doi.org/10.1093/toxsci/56.2.332
  9. Hiliard, C. A., Armstrong, M. J., Bradt, C. I., Hill, R. B., Greenwood, S. K. and Galloway, S. M. : Chromosome aberrations in vitro related to cytotoxicity of nonmutagenic chemicals and metabolic poisons. Environmenatal and Molecular Mutagenesis 31, 316 (1998) https://doi.org/10.1002/(SICI)1098-2280(1998)31:4<316::AID-EM3>3.0.CO;2-G
  10. Morrissey, R. E., George, J. D., Price, C. J., Tyl, R. W., Marr, M. C. and Kimmel, C. A. : The developmental toxicity of bisphenol A in rats and mice. Fundam. Appl. Toxicol. 8, 571 (1987) https://doi.org/10.1016/0272-0590(87)90142-4
  11. Dechaud, H., Ravard, C., Claustrat, F., Aude Brac de la perriere and Pugeat, M. : Xenoestrogen interaction with human sex hormone-binding globulin (hSHBG). Steroids 64, 328 (1999) https://doi.org/10.1016/S0039-128X(98)00114-7
  12. Farabollini, F., Porrini, S., Seta, D. D., Bianchi, F. and DessiFulgheri, F. : Effects of perinatal exposure to bisphenol A on sociosexual behavior of female and male rats. Environmental Health Perspectives 110, 409 (2002) https://doi.org/10.1289/ehp.02110s3409
  13. Pyo, M. Y and Byun, J. A. : Effects of acute oral administration of bisphenol A on the immune function in mice. Yakhak Hoeji 45, 55 (2001)
  14. Lutz, M. B., Kukutsh, N., Ogilvie, A. L. J., Rossner, S., Koch, F., Romani, N. and Schuler, G. : An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Meth. 223, 77 (1999) https://doi.org/10.1016/S0022-1759(98)00204-X
  15. Bach, F. H. and Voynow, N. K. : One-way stimulation in mixed leukocyte cultures. Science 153, 545 (1966) https://doi.org/10.1126/science.153.3735.545
  16. Dolye, A., Griffiths, J. B., Newell, D. G. and Cell & Tissue Culture ; Laboratory procedures, John Wiily & Sons Ltd., U.K. (1993)
  17. Roh, J. K., Chung, H. C., Koh, E. H., Lee, W. Y., Hahn, J. S. and Kim, B. S. : In vitro cytotoxicity of various anticancer drugs to short-term cultured gastric adenocarcinoma cell lines. J. of Korean Cancer Association 23, 3 (1991)
  18. Roitt, I., Brostoff, J. and Male, D. : Immunology, Gower Medical Publishing. London . New York (1985)
  19. Jontell, M., Hanks, C. T., Bratel, J. and Bergenholtz, G. : Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp. J. Dent. Res. 74, 1162 (1995) https://doi.org/10.1177/00220345950740050401
  20. Yamashita, U., Sugiura, T. and Kuroda, E. : Effect of endocrine disruptors on immune reponses in vitro. J. UOEH 24, 1 (2002) https://doi.org/10.7888/juoeh.24.1
  21. Yoshino, S., Ohsawa, M., Sakabe, K., Sagai, M. and Mori, Y. : Influence of bisphenol A on the immune system XXVth symposium on toxicology and environmental health 3rd forum on pharmaceutical health science. P-71, 102 (1999)