Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Enzyme-linked Immunosorbent Assays for Antibodies against Chlamydia Pneumoniae Compared with Microimmunofluorescence Test with Patients with Chronic Cough

만성 기침 환자에서 혈청 클라미디아 항체에 대한 ELISA와 microimmunofluorescence 검사의 비교

  • Lee, Hui Young (Department of Internal Medicine, College of Medicine, Kangwon National University and Clinical Research Institute, Kangwon National University Hospital) ;
  • Kim, Woo Jin (Department of Internal Medicine, College of Medicine, Kangwon National University and Clinical Research Institute, Kangwon National University Hospital)
  • 이희영 (강원대학교 의과대학 내과학교실, 강원대학교병원 임상의학연구소) ;
  • 김우진 (강원대학교 의과대학 내과학교실, 강원대학교병원 임상의학연구소)
  • Received : 2005.01.26
  • Accepted : 2005.06.28
  • Published : 2005.07.30
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Abstract

Background : Chlamydia pneumoniae is a clinically important pathogen, the diagnosis of such infection being based mainly on serology. Microimmunofluorescence (MIF) is the current standard diagnostic method, but is subjective and time-consuming, so the authors tested the serology of chronic cough patients using an EILSA method for the Chlamydial antibody, which is a more objective method, and compared the results with those of the standard method. Method : Thirty-five patients, who visited Kangwon National University Hospital between August 2003 and July 2004, were evaluated. A MIF and ELISA tests were used to determine C. pneumoniae antibody titers. Nasopharyngeal aspirates were examined by polymerase chain reaction (PCR). The Spearman rank correlation test was used for data analysis. Results : Sensitivities of ELISA for IgG, IgA and IgM, as judged by MIF, were 84.0, 84.0 and 40.0% and the specificities were 60.0, 60.0 and 96.7%, respectively. Three patients were Chlamydia PCR positive. Conclusion : ELISA can be a useful tool for studying the seroprevalence of Chlamydia pneumoniae. However, further studies will be required prior to its clinical use.

연구배경 : 클라미디아 폐렴균은 임상적으로 중요한 균으로 혈청검사가 진단에 중요하다. 현재 이용되는 micro-immunofluorescence법은 주관적이고 시간이 많이 소요되므로 좀더 객관적으로 진단할 수 있는 ELISA법을 이용하여 만성 기침을 주소로 내원한 환자들의 혈청을 대상으로 검사하고 표준방법과 결과를 비교하였다. 방 법 : 2003년 8월부터 2004년 7월까지 강원대학교병원을 방문한 35명의 성인 환자에서 얻은 혈청을 ELISA법과 micoimmunofluorescence법으로 시행하였다. 비인두 스왑에서 PCR을 시행하였다. 두 방법의 비교는 비모수 상관계수 분석(Spearman)을 하였다. 결 과 : ELISA 검사결과 IgG는 민감도 84.0%, 특이도 60.0%, IgA는 민감도 84.0%, 특이도60.0%, IgM은 민감도 40.0%, 특이도 96.7%의 결과를 보였다. PCR은 3명의 환자에서 양성이었다. 결 론 : ELISA법이 많은 환자들을 대상으로 하는 역학적 연구에서는 좀더 유용하게 이용될 수 있을 것으로 사료된다. 임상적으로 적용되기 위해서는 좀더 연구가 필요할 것으로 사료된다.

Keywords

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