Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Contamination patterns of Listeria spp. in pork processing plants using random amplified polymorphic DNA

RAPD를 이용한 돈육 가공장의 Listeria 오염양상 분석

  • Ha, Sung-Yeol (Department of Veterinary Medicine, Kangwon National University) ;
  • Choi, Weon-Sang (Department of Biotechnology, College of Natural Science, Dongguk University) ;
  • Bahk, Gyung-Jin (HACCP Team, Korea Health Industry Development Institute) ;
  • Hong, Chong-Hae (Department of Veterinary Medicine, Kangwon National University)
  • Accepted : 2005.08.17
  • Published : 2005.09.22
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Abstract

This study was carried out to understand the contamination patterns of Listeria in pork processing plants. A total of 402 samples were collected from carcass, pork during processing, surfaces of equipment and environment, and 238 isolates of Listeria species were identified. L. innocua was found in 64.7% of the isolates, L. monocytogenes in 33.2%, and L. welshimeri in 2.1%. Random amplified polymorphic DNA (RAPD) analysis performed to investigate the origin and routes of Listeria contamination, showed 21 composite types of L. monocytogenes and 26 composite types of L. innocua. It was confirmed that Listeria contamination begins with contaminated incoming carcass and ever-present contaminants in the processing environments. The persistence and dissemination of the same strain of L. monocytogenes and L. innocua throughout the processing line revealed that the sanitation standard operating procedure should be implemented to minimize the risk of colonization in the workplace. Molecular subtyping of L. innocua allowed us to tracing the possibility of cross-contamination during processing.

Keywords

References

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