Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Solubilization of Rock Phosphates by Alginate Immobilized Cells of Pantoea agglomerans

Alginate에 고정화된 Pantoea agglomerans에 의한 인광석 가용화

  • Ryu, Jeoung-Hyun (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Madhaiyan, Munusamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Seshadri, Sundaram (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2005.06.11
  • Accepted : 2005.07.28
  • Published : 2005.08.30
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Abstract

Pantoea agglomerans immobilized in alginate solubilized four different rock phosphates efficiently under in vitro conditions. The solubilization pattern differed according to the rock phosphate source, where maximum solubilization of Morocco and Tunisia rock phosphates (215.6 and $186.1mg\;P\;L^{-1}$) on 6 days, Israel rock phosphate ($60.98mg\;P\;L^{-1}$) and tricalcium phosphate ($132.3mg\;P\;L^{-1}$) on 10 days and China rock phosphate ($48.8mg\;P\;L^{-1}$) on 12 days after inoculation was observed. The shelf life of the immobilized bacteria immobilized beads stored in two different temperatures was studied for six months. Beads stored at both room temperature as well as cold storage ($4^{\circ}C$) were found equally good in supporting the bacterial population as well as phosphate solubilizing activity. P. agglomerans immobilized in alginate might be exploited for large scale biosolubilization of rock phosphates intended for fertilizer use.

본 실험은 인산가용화균인 Pantoea agglomerans를 alginate를 이용하여 고정화 시킨 후 다양한 종류의 인광석을 대상으로 인산 가용화능을 평가하고 고정화된 균의 보관 및 저장온도에 따른 인산 가용화능의 변화를 살펴보기 위하여 수행되었다. 실험에 사용된 원산지가 다른 인광석 (Tunisia, Israel, Morocco, China) 중 Morocco산 인광석의 가용화 속도가 빠르고 높은 농도의 인산이 가용화 되었다. 반면, 중국산 인광석은 가용화 속도가 느리며, 가용화된 인산의 농도도 매우 낮았다. 고정화된 균의 저장 온도는 6개월간 보관 시 인산 가용화능에 유의성 있는 차이를 나타내자 않았다.

Keywords

References

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