훼손된 산불토양의 미생물다양성

Microbial Diversity in the Soil Damaged by a Forest Fire

산불로 훼손된 토양 미생물군집의 생화학적, 유전적 다양성을 비교하기 위하여 2000년에 산불이 일어났던 강릉지역에서 자연복원지 토양(NS), 인공복원지 토양(AS), 정상토양(NS)을 표토층과 심토층에서 채집하였다. 생화학적 다양성 분석을 위해 각 시료를 BIOLOG system에 직접 적용하였으며 통계처리(SPSS)를 통해 군집분석을 수행하였다. 또한 군집의 유전적 다양성은 토양 미생물의 16S-rDNA를 증폭하여 DGGE(Denaturing Gradient Gel Electrophoresis)를 이용하여 분석하였다. 자연복원지의 심토와 표토, 정상토양의 표토에서만 70% 이상의 생화학적 다양성이 나타났으나, 유전적 다양성의 경우 모든 시료에서 53%에서 68%의 범위의 상동성을 나타냈다. 이러한 결과는 토양미생물 군집의 생화학적 다양성이 반드시 유전적 다양성을 반영하지 않는다는 것을 보여준다.

Changes of biochemical and genetic diversity of microbial communities in the soil damaged by a forest fire were analyzed. Soil samples were collected from Gangnung area where a forest fire was broken out in 2000. Two soil samples were from the burnt area, one from the naturally restoring soil (NS) and the other from the artificially restoring soil (AS). A normal, unaffected soil sample (US) was also included as a control. For the biochemical diversity, each sample was directly applied to the BIOLOG system, and the cluster analysis through a statistic process (SPSS) were performed. Genetic diversity was analyzed through DGGE using 16S-rDNA amplified from soil DNA. Among the samples tested, top soils of US and NS, and sub soil of NS revealed more than 70% of the similarity value in biochemical diversity. In case of genetic diversity, however, the similarity value was found to be in the range of 53% to 68% in all samples. This result indicates that the biochemical diversity is not always correlated with the genetic diversity in the analysis of microbial communities.