Journal of Preventive Medicine and Public Health

The Korean Society for Preventive Medicine (대한예방의학회)
권호 표지

Effects of Bisphenol A on the Placental Function and Reproduction in Rats

Bisphenol A가 흰쥐의 태반 기능과 출산에 미치는 영향

  • Lee, Chae-Kwan (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Seog-Hyun (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Moon, Deog-Hwan (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Jeong-Ho (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Son, Byung-Chul (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Dae-Hwan (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Lee, Chang-Hee (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Hwi-Dong (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Jung-Won (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Jong-Eun (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University) ;
  • Lee, Chae-Un (Institute of Industrial Medicine & Department of Occupational & Environmental Medicine, Busan Paik Hospital, Inje University)
  • 이채관 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김석현 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 문덕환 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김정호 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 손병철 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김대환 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 이창희 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김휘동 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김정원 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 김종은 (인제대학교 산업의학연구소 및 부산백병원 산업의학과) ;
  • 이채언 (인제대학교 산업의학연구소 및 부산백병원 산업의학과)
  • Published : 2005.09.01
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Abstract

Objectives : The aim of this study was to investigate the effects of bisphenol A (BPA), an estrogen-like environmental endocrine disrupter, on the placental function and reproduction in rats. The mRNA levels of the placental prolactin-growth hormone(PRL-GH) gene family, placental trophoblast cell frequency and reproductive data were analyzed. Methods : The pregnancies of F344 Fisher rats ($160g{\pm}20g$) were detected by the presence of the copulatory plug or sperm in the vaginal smear, which marked Day 0 of pregnancy. Pregnant rats were divided into three groups. The control group was intraperitoneally injected with a sesame oil vehicle. The two remaining groups were injected with 50 or 500 mg/kg B.W/day of BPA, resuspended in sesame oil, on either days 7 to 11 or 16 to 20 of pregnancy, with the rats sacrificed on either day 11 or 20, respectively. The mRNA levels of PRL-GH and Pit-1a and b isotype genes were analyzed by Northern blot hybridization and reverse transcription-polymerase chain reaction. The hormone concentrations were analyzed by radioimmunoassay, and the frequency of the placental trophoblast cells observed by a histochemical study. Reproductive data, such as the placental weight and litter size, were surveyed on day 20. The fetal weight was surveyed for 4 weeks after birth. A statistical analysis was carried out using the SAS program (version 8.1). Results : The mRNA levels of the PRL-GH gene family, such as placental lactogen I, Iv and II, prolactin like protein A, C and Cv, and decidual prolactin-related protein were significantly reduced due to BPA exposure. The mRNA levels of the Pit-1a and b isotype genes, which induce the expression of the PRL-GH gene family in the rat placenta, were also reduced due to BPA exposure. The PL-Iv and PL-II concentrations were reduced in the BPA exposed group. During the middle to last stage of pregnancy (Days 11-20), a high dose of BPA exposure reduced the frequency of spongiotrophoblast cells, which are responsible for the secretion of the PRL-GH hormones. Reproductive data, such as the placental and fetal weights and the litter size, were reduced, but that of the pregnancy period was extended in the BPA exposed compared to the control group. Conclusions : BPA disrupts the placental functions in rats, which leads to reproductive disorders.

Keywords

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