Journal of Yeungnam Medical Science
- Volume 22 Issue 2
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- Pages.166-182
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- 2005
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- 2799-8010(eISSN)
Activation and Abnormalities of Cell Cycle Regulating Factor in Head and Neck Squamous Cell Carcinoma Cell Lines: Abnormal Expression of CDKN2 Gene in Laryngeal Squamous Cell Carcinoma
두경부 편평상피세포암 세포주에서 세포주기조절인자의 활성 및 이상 : 후두편평상피세포암에서 종양억제유전자 CDKN2 유전자의 발현이상
- Song, Si-Youn (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
- Han, Tae-Hee (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
- Bai, Chang-Hoon (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
- Kim, Yong-Dae (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University) ;
- Song, Kei-Won (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University)
- 송시연 (영남대학교 의과대학 이비인후과학교실) ;
- 한태희 (영남대학교 의과대학 이비인후과학교실) ;
- 배창훈 (영남대학교 의과대학 이비인후과학교실) ;
- 김용대 (영남대학교 의과대학 이비인후과학교실) ;
- 송계원 (영남대학교 의과대학 이비인후과학교실)
- Published : 2005.12.30
Abstract
Background: Cyclin-dependent kinase (CDK) inhibitors are family of molecules that regulate the cell cycle. The CDKN2, a CDK4 inhibitor, also called p16, has been implicated in human tumorigenesis. The CDKN2 inhibits the cyclin/CDK complexes which regulate the transition from G1 to S phase of cell cycle. There is a previous report that homozygous deletion of CDKN2 region on chromosome 9p21 was detected frequently in astrocytoma, glioma and osteosarcoma, less frequently in lung cancer, leukemia and ovarian cancer, but not detected in colon cancer and neuroblastoma. However, little is known about the relationship between CDKN2 and laryngeal cancer. Therefore this study was initiated to investigate the role of CDKN2 in human laryngeal squamous cell carcinoma development.1) Materials and methods: We used 5 human laryngeal carcinoma cell lines whether they have deletions or losses of CDKN2 gene expression by DNA-PCR or RT-PCR, respectively. We examined 8 fresh frozen human laryngeal cancer tissues to detect the loss of heterozygosity (LOH) of CDKN2. PCR was performed by using microsatellite markers of short arm of human chromosome 9 (D9S126, D9S144, D9S156, D9S161, D9S162, D9S166, D9S171, D9S200 and D9SIFNA). For informative cases, allelic loss was scored if the signal of one allele was significantly decreased in tumor DNA when compared to the same allele in normal DNA. Results: The CDKN2 DNA deletion was observed in 3 cell lines. The CDKN2 mRNA expression was observed in only one cell line, which was very weak. LOH was detected in 7 cases (87.5%). Conclusion: These results suggest that CDKN2 plays a role in the carcinogenesis of human laryngeal squamous cell carcinoma.
정상인의 말초혈액 림프구 DNA를 주형으로 사용하여 DNA PCR을 시행하였다. 그 결과 5례 모두에서 예상되는 167bp 크기의 CDKN2 genomic DNA 단편이 증폭됨을 관찰할 수 있었다. 정상인의 말초혈액 림프구로부터 분리한 mRNA를 사용하여 cDNA를 합성하고 이를 주형으로 사용하여 RT-PCR와 시행하였다. 그 결과 예상되는 355bp 및 468bp의 CDKN2 및