Journal of Periodontal and Implant Science

  • 제35권2호
  • /
  • Pages.427-436
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  • 2005
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

E. coli lipopolysaccharides로 유도된 사람 호중구에서 CD14, Toll-like receptors, cytoskeletal inhibitors 그리고 $NF-{\kappa}B$ inhibitor가 MMP-8 분비에 미치는 영향

Effect of CD14, Toll-like receptors, cytoskeletal inhibitors and $NF-{\kappa}B$ inhibitor on MMP-8 release from human neutrophils induced by E. coli lipopolysaccharides.

  • 양승민 (서울대학교 치과대학 치주과학교실, 성균관대학교 의과대학 치과학교실, 삼성서울병원 치과진료부 치주과) ;
  • 김태일 (서울대학교 치과대학 치주과학교실) ;
  • 설양조 (서울대학교 치과대학 치주과학교실) ;
  • 이용무 (서울대학교 치과대학 치주과학교실) ;
  • 구영 (서울대학교 치과대학 치주과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 류인철 (서울대학교 치과대학 치주과학교실)
  • Yang, Seung-Min (Department of Periodontology, School of Dentistry, Seoul National University, Department of Dentistry, School of Medicine, Sungkyunkwan University, Department of Periodontics, The institute of Oral Health Science, Samsung Medical Center) ;
  • Kim, Tae-Il (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of Dentistry, Seoul National University)
  • 발행 : 2005.06.30
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초록

Objective: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. Material and methods: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. Results: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti-CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of microtubule and microfilament polymerization significantly decreased E-LPS-induced MMP-8release. TPCK inhibited E-LPS-induced MMP-8 release. Conclusion: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and $NF-{\kappa}B$ pathway.

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