Journal of Periodontal and Implant Science

  • 제35권2호
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  • Pages.289-298
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  • 2005
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

치주인대세포에 대한 Bone morphogenetic protein-7의 영향

Effect of BMP-7 on the rat periodontal ligament cell

  • 김경희 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 김영준 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 정현주 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소)
  • Kim, Kyung-Hee (Department of Periodontology, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Young-Jun (Department of Periodontology, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Department of Periodontology, Dental Science Research Institute, Chonnam National University)
  • 발행 : 2005.06.30
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초록

Bone morphogenetic protein-7(BMP-7), a member of the transforming growth factor superfamily, stimulates osteoblast differentiation and bone formation. There are lots of evidences supporting a direct participation of periodontal ligament(PDL) cells on periodontal tissue regeneration. The purpose of this study was to evaluate the effect of recombinant human(rh) BMP-7 on primary rat PDL cells in vitro, with special focus on the ability of bone formation. The PDL cells were cultured with rhBMP-7 at the concentration of 0, 10, 25, 50, 100 and 200ng/ml for MTT assay. We evaluated the alkaline phosphatase activity at 3 and 5 days of incubation and the ability to produce mineralized nodules of rat PDL cells at 14 days of cell culture in concentration of 0, 10, 25, 50 and 100ng/ml. The cell activity was not reduced in cells treated with BMP-7 at $10{\sim}100ng/ml$, whereas the cell activity was reduced in the concentration of 200ng/ml than the control at day 1 and 3(p<0.01). At 3 and 5 day, alkaline phosphatase activity was significantly increased in cells treated with BMP-7 at 50ng/ml and 100ng/ml(p<0.05). The area of mineralized bone nodule was greater in cells treated with BMP-7 at 50 and 100 ng/ml than the control(p<0.01). These results suggest that rhBMP-7 stimulate rat PDL cells to differentiate toward osteoblast phenotype and secretion of the extracellular matrix of rat PDL cells.

키워드

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