Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effects of biphasic calcium phosphate on bone formation in human fetal osteoblasts

Biphasic Calcium Phosphate가 태아골모세포의 골 형성에 미치는 영향

  • Shin, Kye-Chul (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Jang, Kil-Young (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lee, Myoung-Ku (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Yoon, Ho-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Song, Jae-Bong (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Hyun-A (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 신계철 (원광대학교 치과대학 치주과학교실) ;
  • 장길용 (원광대학교 치과대학 치주과학교실) ;
  • 이명구 (원광대학교 치과대학 치주과학교실) ;
  • 윤호상 (원광대학교 치과대학 치주과학교실) ;
  • 송제봉 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 피성희 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2005.03.30
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Abstract

목적 : 이 연구의 목적은 치과 영역에서 골 재생을 촉진하기 위해, 현재 많이 사용하고 있는 BDX(bovinederived xenograft)와 비교하여 BCP(biphasic calcium phosphate)의 효과를 알아보기 위함이다. 실험 재료 및 방법: 본 연구는 태아골모세포주(hFOB 1.19)를 사용하였으며, 사용된 골 이식재에 따라 2개의 실험군으로 구분하였고, 각 실험에 적절한 농도의 BDX와 BCP를 첨가하였다. 그리고, 세포 증식도 검사, 교원질 합성량 분석, 염기성 인산분해효소 활성도 측정, Western blot 분석을 통한 OC과 BSP의 발현 정도등의 실험을 진행하였다. 결과 : BDX와 BCP는 대조군과 비교하여 세포 증식에서 유의한 차이가 없었지만, 교원질 합성량, 염기성 인산분해효소의 활성, 그리고 OC과 BSP의 발현에 있어 대조군과 비교하여 유의하게 증가를 보였다. 그러나, 두 이식재간의 유의한 차이는 보이지 않았다. 결론 : 본 실험실적 연구에서 BCP는 골모세포분화에 긍정적인 영향을 미침으로써 효과적인 이식재로 사용할 수 있음을 가늠할수 있었다.

Keywords

References

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