한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제20권1호
  • /
  • Pages.43-47
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  • 2005
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지

유통 중인 신선 채소류의 미생물 오염도 평가

Microbial Contamination Levels of Fresh Vegetables Distributed in Markets

  • Choi Jin-Won (Department of Food Science and Technology, Chung-Ang University) ;
  • Park Shin Young (Department of Food Science and Technology, Chung-Ang University) ;
  • Yeon Ji-Hye (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee Min Jeong (Department of Food Science and Technology, Chung-Ang University) ;
  • Chung Duck Hwa (Gyeongsang National University) ;
  • Lee Kyu-Ho (Hankuk Univ. of Foreign Studies) ;
  • Kim Min-Gon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Dong-Ha (Korea Food and Drug Administration) ;
  • Kim Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha Sang-Do (Department of Food Science and Technology, Chung-Ang University)
  • 발행 : 2005.03.01
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초록

본 연구는 신선채소류의 대형매장과 재래시장에서의 청결도를 비교하기 위하여 시중에 유통 중인 상추, 깻잎, 오이를 서울과 경기남부의 15개 대형매장과 21개 재래시장에서 sampling하여 총호기성균, 대장균군, Escherichia coli 및 Listeria monocytogenes의 오염도 수준을 평가하였다. 대형매장과 재래시장에서의 상추, 깻잎, 오이의 총호기성균과 대장균군의 오염도는 유의차가 없었다(p>0,05).그러나 3가지 채소에서의 총호기성균 오염수준은 상추, 깻잎, 오이 순으로 나타났으며 그 수준은 각각 평균 $7.01{\pm}0.14\;log_{10}CFU/g$(대형)과 $7.10{\pm}0.11\;log_{10}CFU/g$(재래), $6.69{\pm}0.20\;log_{10}CFU/g$(대형)과 $6.44{\pm}0.13\;log_{10}CFU/g$(재래), $5.37{\pm}0.25\;log_{10}CFU/g$(대형)과 $5.27{\pm}0.19\;log_{10}CFU/g$(재래)이었으며 대장균군의 오염 순위 또한 같은 양상을 보였다. E. coli 검출률 역시 3가지 채소 모두에서 매장간의 유의적 차이는 없었으며(p>0.05) 상추와 깻잎은 대형매장과 재래시장 모두에서 $30\%$ 이상의 빈도로 검출되었으나, 오이에서는 $10\%$ 미만의 검출빈도를 보였다. L. monocytogenes는 모든 채소에서 검출되지 않았다(ND: 오이 <3 CFU/g, 상추, 깻잎<10 CFU/g). 따라서 본 연구에서 측정된 오염수준은 신선채소류의 미생물 위해평가(MRA)프로그램 개발의 기초자료로 활용될 수 있을 것이다.

This study monitored and compared the contamination levels of total aerobic bacteria, coliforms, Escherichia coli, and L. monocytogenes of either lettuce, sesame leaf, or cucumber sampled from either 15 super markets(SM) or 21 traditional markets(TM) located in both Seoul and the southern part of Gyunggi. Contamination levels of total aerobic bacteria and coliforms in lettuce, sesame leaf, or cucumber from SM or TM were not (p>0.05) significantly different. The highest contamination levels of total aerobic bacteria were observed in lettuce and followed by sesame leaf and cucumber. The contamination levels of total aerobic bacteria in lettuce, sesame leafs, and cucumbers were $7.01{\pm}0.14\;log_{10}CFU/g(SM)$ and $7.10{\pm}0.11\;log_{10}CFU/g(TM)$, $6.69{\pm}0.20\;log_{10}CFU/g(SM)$ and $6.44{\pm}0.13\;log_{10}CFU/g(TM)$, and $5.37{\pm}0.25 \;log_{10}CFU/g(SM)$ and $5.27{\pm}0.19\;log_{10}CFU/g(TM)$, respectively. A similar pattern of contamination rank was observed with the coliforms in three vegetables as was observed with the total aerobic bacteria E. coli were not significantly (p>0.05) different between SM and TM and isolated over $30\%$ in lettuce and sesame leaf and below $10\%$ in cucumbers. L. monocytogenes were not detected in all three vegetables(ND: cucumber <3 CFU/g, lettuce and sesame leaf <10 CFU/g). The microbial contamination levels determined in the present study may be used as the primary data to execute microbial risk assessment of fresh vegetables.

키워드

참고문헌

  1. 박희옥, 김창민, 우건조, 박선희, 이동하, 장은정, 박기환: 최근 한국에서 발생한 식중독 모니터링 및 추이 분석, 한국식품위생안전성학회지, 16, 280-294 (2001)
  2. Itoh, Y., Sugita-Konishi, Y. Kasuage, F., Iwaki, M., Hara- Kudo, Y., Saito, N., Noguchi, Y., Konuma, H. and Kumagai, S.: Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts, Appl. Environ. Microbiol., 64, 1352-1535 (1998)
  3. Hedberg, C.W., Angulo, F.G., White, K.E., Langkop, C.W., Schell, W.L., Stobierski, M.G., Schuchat, A., Besser, J.M., Dietrich, S., Helsel, L., Griffin, P.M., McFarland, J.W., Osterhorm, M.T. and The Investigation Team: Outbreaks of Salmonellosis Associated with Eating Uncooked Tomatoes: Implications for Public Health, Epidemiol. Infect., 122, 385-393 (1999) https://doi.org/10.1017/S0950268899002393
  4. Beuchat, L.R., Harris, L.R., Linda, J., Ward, T.E. and Kajs, T.M.: Development of a Proposed Standard Method for Assessing the efficacy of Fresh Produce Sanitizers. J. Food Prot., 64, 1103-1109 (2001)
  5. Harris, L.J., Beuchat, L.R. Kajs, T.M. Ward, T.E. and Taylor, C.J.: Efficacy and Reproducibility of a Produce Wash in Killing Salmonella on the Surface of Tomatoes Assessed with a Proposed Standard Method for Produce Santizers, J. Food Prot., 64, 1477-1482 (2001)
  6. USDA. U.S. Department of Agriculture/Food Science & Inspection Service, Office of Public Health and Science. Microbiology Laboratory Guidebook. Available from http:// www.fsis.usda.gov. Accessed Oct.25, (2004)
  7. SAS Institute Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (2002)
  8. Luginbuke, R., Schlotzhauer, S.D.: SAS/SAT Guide for Personal Computers, $6^{th}$ ed. SAS Institute, Inc., Cary, NC, USA (1987)
  9. Taormina, P.J., Rocelle, M., Clavero, S. and Beuchat, L.R.: Comparison of Selective Agar Media and Enrichment Broths for Recovering Heat-stressed Escherichia coli O157:H7 from ground beef, Food Microbiol., 15, 631-638 (1998) https://doi.org/10.1006/fmic.1998.0202
  10. Willcox, F., Mercier, M., Hendrickx, M., Tobback, P.: Modelling the influence of temperature and carbon dioxide upon the growth of Psedpmonas fluorescens. Food Microbiol., 10, 159-173 (1993) https://doi.org/10.1006/fmic.1993.1016
  11. Gram, L., Ravn L., Rasch, M., Bruhn, J.B., Christensen, A.B., Givskov, M.: Food spoilage-interactions between food spoilage bacteria. Int. J. Food Microbiol., 78, 79-97 (2002) https://doi.org/10.1016/S0168-1605(02)00233-7
  12. Francis, G.A., Thomas, C. and O'Beirne, D.O. : The microbiological safety of minimally processed vegetables. Int. J. Food Sci. Technol., 34, 1-22 (1999) https://doi.org/10.1016/S0168-1605(96)01167-1
  13. KFDA. Korea Food and Drug Administration. http:// www.kfda.co.kr. Accessed Oct.25 (2004)
  14. Lund, B. M.: The microbiological safety of prepared salad vegetables. Food Technology International Europe, pp. 196- 2000. Institute of food Science and Technology (1993)
  15. Beuchat. L.R., R.E. Brackett, D.Y.Y. Hao and D.E. Conner: Growth and thermal inactivation of Listeria monocytogenes in cabbage and cabbage juice. Can. J. Microbiol., 32, 791-795 (1986) https://doi.org/10.1139/m86-145
  16. Donnelly, C.W. and E.H. Briggs: Psychrotropic growth and thermal inactivation of Listeria monocytogenes as a function of milk composition. J. Food Prot., 49, 994-998 (1986)
  17. Welshimer, H.J.: Isolation of Listeria monocytogenes from vegetation. J. Bacteriol., 95, 300-320 (1968)
  18. Weiss, J. and H.P.R. Seeliger.: Incidence of Listeria monocytogenes in nature. Appl. Environ. Microbiol., 30, 29-32 (1975)
  19. Skovgaard, N. and C.A. Morgan: Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol., 6, 229-242 (1988) https://doi.org/10.1016/0168-1605(88)90015-3
  20. Heisick, J.E., D.E. Wagner, M.L. Nierman and J.T. Peeler: Listeria spp. found on fresh market produce. Appl. Environ. Microbiol., 55. 1925-1927 (1989)
  21. Wehr, M.H.: Listeria monocytogenes-A current dilemma. J. Assoc. Off. Anal. Chem., 70, 769 (1987)