Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Screening of Korean Medicinal Herbs for Hormonal Activities using Recombinant Yeast Assay and MCF-7 Human Breast Cancer Cells

재조합효모와 MCF -7 사람유방암세포주를 이용한 한국산 약용식물의 호르몬 활성 스크리닝

  • Yang Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hong Hee-Do (Korea Food Research institute, Sungnam city) ;
  • Cho Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn Nam-Shik (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Sun bo (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee Seong-Hun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung Ji-Youn (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Choi Changsun (Department of Veterinary Pathology, College of Veterinary Medicine, Ohio State University, USA)
  • 양세란 (서울대학교 수의과대학) ;
  • 홍희도 (한국식품개발연구원) ;
  • 조성대 (서울대학교 수의과대학) ;
  • 안남식 (서울대학교 수의과대학) ;
  • 정지원 (서울대학교 수의과대학) ;
  • 박준석 (서울대학교 수의과대학) ;
  • 조은혜 (서울대학교 수의과대학) ;
  • 황재웅 (서울대학교 수의과대학) ;
  • 순보 (서울대학교 수의과대학) ;
  • 박정란 (서울대학교 수의과대학) ;
  • 이성훈 (서울대학교 수의과대학) ;
  • 정지윤 (서울대학교 수의과대학) ;
  • 최창순 (오하이오 주립대학 수의과대학 병리학교실)
  • Published : 2005.03.01
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Abstract

To investigate whether there are estrogenic and anti-estrogenic activities in various medicinal herbs and discover prominent chemo-preventive agents, we screened and compared the ethanol extracts of 9 plants through the recombinant yeast assay and MCF-7 human breast cancer cell assay, In recombinant yeast assay, seven medicinal herbs showed estrogenicity, and four extracts showed androgenecity. In MCF-7 proliferation assay, the growth of MCF-7 cells was inhibited by eight extracts before and even after co-treatment with bisphenol A. It is interesting that the extracts of Glycyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, and Panax ginseng C.A. Meyer exhibited inhibitory effects as phytoestrogens in estrogen-responsive human breast cancer cells. This study suggests that some Korean medicinal herbs might be considered as phytoestrogens and be useful to further analyze those plants which contain the estrogenic effect in order to identify the active principles.

약용식물내 에스트로겐성과 항-에스트로겐성을 조사하고 항암인자를 발견하기 위하여, 본 연구는 에탄올추출로 제조된 9종류의 한국산 약용식물에 대하여 재조합효모와 MCF-7 사람유방암세포주를 이용하여 스크리닝하고 비교하였다. 재조합효모를 이용한 실험결과, 7종류의 약용식물에서 에스트로겐성이 나타났고, 4종류에서 안드로겐성이 나타났다. 또한 MCF-7 사람유방암세포주를 이용한 실험결과, 8종류의 추출물이 MCF-7 세포의 성장을 억제하는 것으로 확인되었으며 비스페놀 A와 동시 처치한 경우에도 유의적으로 억제하는 것으로 나타났다. 또한 Glycyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, Panax ginseng C.A. Meyer는 식물성 에스트로겐으로서 에스트로겐에 양성인 사람유방암세포의 증식을 유의적으로 억제시키는 흥미로운 결과가 제시되었다. 따라서 이번 연구는 한국산 약용식물이 식물성 에스트로겐과 항암인자로서 이용될 수 있으며, 에스트로겐의 활성을 조사하는데 유용하게 이용될 수 있을 것으로 사료된다.

Keywords

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